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Series GSE246147 Query DataSets for GSE246147
Status Public on Oct 26, 2023
Title Single cell RNAseq of brian cells in the basolateral amygdala with fear conditioning
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Memory encodes past experiences, thereby enabling future plans. The basolateral amygdala (BLA) is a center of salience networks that underlie emotional experiences and thus plays a key role in long-term fear memory formation. Here we used single-cell transcriptomics to illuminate the cellular and molecular architecture of the role of the basolateral amygdala in long-term memory. We identified transcriptional signatures in subpopulations of neurons and astrocytes that were memory-specific and persisted for weeks. These transcriptional signatures implicate neuropeptide and brain-derived neurotrophic factor (BDNF) signaling, mitogen-activated protein kinase (MAPK) and cAMP response element-binding protein (CREB) activation, ubiquitination pathways, and synaptic connectivity as key components of long-term memory. Strikingly, upon long-term memory formation a neuronal sub-population defined by increased Penk and decreased Tac expression constituted the most prominent component of the BLA’s memory engram.
 
Overall design To study the molecular and cellular basis of memory consolidation, we applied fear conditioning paradigm on FosERcre - tdTomato reporter mice and performed single cell RNAseq for the basolateral amygdala.
 
Contributor(s) Sun W, Liu Z, Chen M, Jiang X, Dong H, Südhof T, Quake S
Citation(s) 38326616
Submission date Oct 24, 2023
Last update date Jul 26, 2024
Contact name Wenfei Sun
E-mail(s) Wenfei-sun@stanford.edu
Organization name Stanford University
Street address 318 Campus Dr.
City Stanford
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6361)
GSM7857235 BLA_FearRecall_fr1_A1_S161
GSM7857236 BLA_FearRecall_fr1_A10_S170
GSM7857237 BLA_FearRecall_fr1_A2_S162
Relations
BioProject PRJNA1031658

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE246147_barcodes.tsv.gz 40.5 Kb (ftp)(http) TSV
GSE246147_cell_type_data.csv.gz 32.3 Kb (ftp)(http) CSV
GSE246147_features.tsv.gz 254.2 Kb (ftp)(http) TSV
GSE246147_matrix.mtx.gz 139.3 Mb (ftp)(http) MTX
GSE246147_umap_data.csv.gz 105.7 Kb (ftp)(http) CSV
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Raw data are available in SRA

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