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Series GSE24636 Query DataSets for GSE24636
Status Public on Dec 15, 2011
Title Effect of salicylic acid on Fusarium graminearum, the major causal agent of fusarium head blight in wheat
Organism Fusarium graminearum
Experiment type Expression profiling by array
Summary Salicylic acid (SA) is one of the key signal molecules in regulating plant resistance to diverse pathogens. It is predominantly associated with resistance against biotrophic and hemibiotrophic pathogens, and triggering systemic acquired resistance (SAR) in Arabidopsis. However, whether and how SA directly affects Fusarium graminearum and how SA influences the defence efficiency of wheat against fusarium head blight (FHB) are still poorly understood. Previous experiments have shown that the growth of F. graminearum mycelia and the germination of spores were significantly inhibited, and eventually stopped by increasing amounts of SA in both liquid and solid media cultures. Co-inoculation of SA and Fg spores has led to reduced FHB symptoms in the very susceptible Triticum aestivum cultivar ‘Roblin’. To better understand the effect of SA on F. graminearum mycelial growth, we have compared the expression profiles of SA-treated and untreated F. graminearum liquid cultures after 8 and 24 h of treatment, using an F. graminearum custom-commercial microarray. The microarray analysis suggests that F. graminearum can metabolize SA through two pathways, the gentisate and catechol pathways that are present in many fungal species. Additional experiments have confirmed the capacity of F. graminearum to metabolize SA. Our results demonstrate that, although F. graminearum has the capacity to metabolize SA, SA has a significant and direct impact on F. graminearum through a reduction in efficiency of germination and growth at higher concentrations.
 
Overall design Untreated and Salicylic Acid (SA) treated liquid cultures of F. graminearum at 8h and 24h collection times. Three biological replicates per time point and treatment, 2 technical replicates (dye flips) per sample.
 
Contributor(s) Qi P, Zheng Y, Ouellet T, Johnston A, Harris LJ
Citation(s) 22385623
Submission date Oct 12, 2010
Last update date Mar 22, 2012
Contact name Linda J Harris
E-mail(s) Linda.Harris@agr.gc.ca
Phone 613-759-1314
Organization name Agriculture & Agri-Food Canada (AAFC)
Department Eastern Cereal & Oilseed Research Centre
Lab Building #21
Street address 960 Carling Ave.
City Ottawa
State/province Ontario
ZIP/Postal code K1A 0C6
Country Canada
 
Platforms (1)
GPL11046 Fusarium graminearum 4x44k
Samples (12)
GSM607560 Fg_8h_BR1_cy5
GSM607561 Fg_8h_BR2_cy5
GSM607562 Fg_8h_BR3_cy5
Relations
BioProject PRJNA132471

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24636_RAW.tar 53.0 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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