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Series GSE246709 Query DataSets for GSE246709
Status Public on Nov 05, 2023
Title Single-nucleus transcriptomics of epicardial adipose tissue from female pigs reveals effects of exercise training on resident innate and adaptive immune cells
Organism Sus scrofa
Experiment type Expression profiling by high throughput sequencing
Summary Coronary artery disease (CAD) is a leading cause of death in women. Although exercise mitigates CAD, the mechanisms by which exercise impacts epicardial adipose tissue (EAT) are unknown. We hypothesized that exercise promotes an anti-inflammatory microenvironment in EAT from female pigs. Female Yucatan pigs (n=7) were assigned to sedentary (SED) or exercise (EX) treatments and coronary arteries were occluded (O vs N) with an ameroid to mimic CAD. EAT was collected for bulk and single-nucleus transcriptomic sequencing (snRNA-seq). Clustering analysis revealed nine cell types in EAT with the fibroblast and macrophage populations predominantly from O-EX EAT and the T cell population predominantly from the N-EX EAT. Exercise upregulated G-protein coupled receptor, S100 family, and FAK pathways and downregulated the coagulation pathway. Exercise caused increased interaction between immune cells and endothelial and mesenchymal cells in the insulin-like growth factor pathway and between endothelial cells and other cell types except B cells in the platelet endothelial cell adhesion molecule pathway 1. Coronary occlusion impacted the largest number of genes in T and endothelial cells. Genes related to fatty acid metabolism were the most highly upregulated in non-immune cells from O-EX EAT. Sub-clustering of endothelial cells revealed that N-EX EAT separated from other treatments. In conclusion, exercise training increased interaction amongst immune and mesenchymal and endothelial cells in female EAT. Exercise was minimally effective at reversing alterations in gene expression in endothelial and mesenchymal cells in EAT surrounding occluded coronary arteries. These findings lay the foundation for future work focused on the impact of exercise on cell types in EAT from women.
Overall design Animals were divided into two exercise (sedentary vs exercised) and two coronary artery (non-occluded and occluded) treatment groups. Surgeries were performed to place an ameroid constrictor (2.5-3.5 mm) around the proximal left circumflex artery. The ameroid constrictor progressively narrowed the artery and induced an inflammatory response resulting in total obstruction occurring at approximately two-three weeks post-operatively. Pigs recovered for eight weeks before the exercise training experimental regimen was initiated and maintained for 14 weeks. Speed and duration of the exercise training sessions were progressively increased so that during the last three weeks of training, animals ran at 4-5.5 mph for 60 minutes and at 6 mph for 5-15 minutes. Sedentary pigs maintained normal activity in their pens throughout the experimental protocol.
Contributor(s) Ahmad I, Gupta S, Faulkner P, Mullens D, Thomas M, Sytha SP, Ivanov I, Cai JJ, Heaps CL, Newell-Fugate AE
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NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL139903 Exercise training-enhanced reactive oxygen species as protective mechanisms in the coronary microcirculation TEXAS A&M UNIVERSITY Cristine L Heaps
BioProject PRJNA1034048
Submission date Oct 31, 2023
Last update date Nov 06, 2023
Contact name Annie Newell-Fugate
Organization name Texas A&M University
Department Department of Veterinary Physiology & Pharmacology
Street address 4466 TAMU
City College Station
State/province TX
ZIP/Postal code 77843-4466
Country USA
Platforms (1)
GPL26351 Illumina NovaSeq 6000 (Sus scrofa)
Samples (11)
GSM7875115 EAT, Exercise, Non-occluded; N1_Landrace
GSM7875116 EAT, Sedentary, Non-occluded; N2_Landrace
GSM7875117 EAT, Exercise, Occluded; O1_Landrace

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Supplementary file Size Download File type/resource
GSE246709_EAT_agg_filtered_feature_bc_matrix.h5 61.1 Mb (ftp)(http) H5
GSE246709_PWAT_Bulk_Raw_counts.csv.gz 483.1 Kb (ftp)(http) CSV
GSE246709_RAW.tar 65.3 Mb (http)(custom) TAR (of H5)
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