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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 05, 2023 |
| Title |
Docetaxel inhibits epithelial-mesenchymal transition in human mammary cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Epithelial–mesenchymal transition (EMT) is a reversible and dynamic biological process in which epithelial cells acquire mesenchymal characteristics including enhanced stemness and migratory ability. EMT can facilitate cancer metastasis and is a known driver of cellular resistance to common chemotherapeutic drugs such as docetaxel. Current chemotherapeutic practices such as docetaxel treatment can promote EMT and increase the chance of tumour recurrence and resistance, calling for new approaches in cancer treatment. Here we show that prolonged docetaxel treatment at a sub-IC50 concentration inhibits EMT in immortalized human mammary epithelial (HMLE) cells.
Using immunofluorescence, flow cytometry, and bulk transcriptomic sequencing to assess EMT progression, we analysed a range of cellular markers of EMT in docetaxel-treated cells and observed an upregulation of epithelial markers and downregulation of mesenchymal markers in the presence of docetaxel. This finding suggests that docetaxel may have clinical applications not only as a cytotoxic drug but also as an inhibitor of EMT-driven metastasis and multidrug resistance, depending on the concentration of its use.
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| Overall design |
Comparative gene expression profiling using RNA-seq data for HMLE cells at 0 and 20 d upon exposure to EMT induction either with or without docetaxel.
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| Web link |
https://doi.org/10.1021/acs.molpharmaceut.3c00425
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| Contributor(s) |
Beerkens SJ, King JJ, Irving KL, Bhatia S, Thompson EW, Smith NM, Iyer KS, Evans CW |
| Citation(s) |
38029291 |
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| Submission date |
Nov 04, 2023 |
| Last update date |
Feb 08, 2024 |
| Contact name |
Jessica King |
| E-mail(s) |
jessicaking736@gmail.com, jessica.king@research.uwa.edu.au
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| Organization name |
University of Western Australia
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| Department |
School of Molecular Sciences
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| Street address |
35 Stirling Hwy
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| City |
Crawley |
| State/province |
WA |
| ZIP/Postal code |
6009 |
| Country |
Australia |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
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| GSM7882306 |
Docetaxel-treated 20 d, biological replicate 2 |
| GSM7882307 |
Untreated 0 d, biological replicate 2 |
| GSM7882308 |
Untreated 20 d, biological replicate 2 |
| GSM7882309 |
TGF-β-treated 20 d, biological replicate 2 |
| GSM7882310 |
Spontaneous EMT 20 d, biological replicate 3 |
| GSM7882311 |
Docetaxel-treated 20 d, biological replicate 3 |
| GSM7882312 |
Untreated 0 d, biological replicate 3 |
| GSM7882313 |
TGF-β-treated 20 d, biological replicate 3 |
| GSM7882314 |
Spontaneous EMT 20 d, biological replicate 4 |
| GSM7882315 |
Docetaxel-treated 20 d, biological replicate 4 |
| GSM7882316 |
Untreated 0 d, biological replicate 4 |
| GSM7882317 |
TGF-β-treated 20 d, biological replicate 4 |
| GSM7882318 |
Mesenchymal, biological replicate 1 |
| GSM7882319 |
Mesenchymal, biological replicate 2 |
| GSM7882320 |
Mesenchymal, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA1035779 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE247028_RAW.tar |
3.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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