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Series GSE247884 Query DataSets for GSE247884
Status Public on Dec 01, 2023
Title Identifying molecular targets of endogenous ADAR3 in gliolastoma cells
Organism Homo sapiens
Experiment type Other
Summary Through immunoprecipitation of epitope-tagged ADAR3 protein, several ADAR3-bound RNAs have been reported till date. However, exogenous expression of proteins are usually associated with altered gene expression profiles and thus, can influence binding profiles of RNA-binding proteins (RBPs) being studied. Here, we used affinity-purified antibody to pull down ADAR3 endogenously expressed in U373 glioblastoma cells and identified transcripts significantly enriched in the ADAR3 immunoprecipitants over control. To serve as control, antisera isolated from rabbit prior to immunization with ADAR3 antigen was used to perform the IP.
Overall design RNA Immunoprecipitation-Sequencing (RIP-Seq) for endogenously-expressed ADAR3 protein in U373 glioblastoma cells.
Contributor(s) Mukherjee P, Hundley HA
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Submission date Nov 15, 2023
Last update date Dec 01, 2023
Contact name Heather Ann Hundley
Organization name Indiana University
Department Biology
Lab Hundley Lab
Street address 1001 E. 3rd Street
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM7902669 U373, Input, Rep 1
GSM7902670 U373, Input, Rep 2
GSM7902671 U373, Input, Rep 3
BioProject PRJNA1040867

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Supplementary file Size Download File type/resource
GSE247884_U373_Input.csv.gz 1.6 Mb (ftp)(http) CSV
GSE247884_U373_RIPSeq.csv.gz 2.4 Mb (ftp)(http) CSV
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