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Series GSE248166 Query DataSets for GSE248166
Status Public on Dec 01, 2023
Title Analysis of gene expression changes in lipid droplet formation of human liver cancer cell HepG2
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Fatty liver is a condition of excessive triglyceride accumulation in hepatocytes. Additionally, hepatocytes showed a high degree of fat droplet accumulation during excessive alcohol consumption and metabolic syndrome. However, the molecular mechanisms involved in fat droplet formation remain unknown. This study used an in vitro fatty liver formation model of the human liver cancer cell line HepG2 to comprehensively search for fat droplet formation-related genes whose expression changes during fat droplet formation. Microarray analysis with extracted total RNA determined the genes that are involved in fat droplet formation and confirmed their expression by real-time polymerase chain reaction after culturing HepG2 cells in a culture medium containing 0, 50, 200, and 500 μM of oleic acid for 24 h. The results revealed 142 genes demonstrating increased expressions by >2.0 fold with oleic acid treatment and 426 genes demonstrating decreased expressions. Perilipin 2 (PLIN2) was estimated as the gene most closely associated with fatty liver. Lipid droplet formation in HepG2 upregulated PLIN2 in an oleic acid-treated concentration-dependent manner. These results indicate the involvement of genes in oleate treatment-induced lipid droplet formation in HepG2, particularly PLIN2 may play an important role.
Overall design Gene expressions in the HepG2 cell line treated with 0, 50, 200, and 500 μM of oleic acid were examined by microarray analysis.
Contributor(s) Chiba M, Matsumoto K
Citation(s) 38283130
Submission date Nov 17, 2023
Last update date Jan 31, 2024
Contact name Mitsuru Chiba
Phone +81172395965
Organization name Hirosaki University
Department Graduate School of Health Sciences
Lab Department of Bioscience and Laboratory Medicine
Street address 66-1 Hon-cho
City Hirosaki
State/province Aomori
ZIP/Postal code 036-8564
Country Japan
Platforms (1)
GPL16699 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)
Samples (8)
GSM7906698 0 μM_oleic_acid_rep1
GSM7906699 0 μM_oleic_acid_rep2
GSM7906700 50 μM_oleic_acid_rep1
BioProject PRJNA1041859

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Supplementary file Size Download File type/resource
GSE248166_RAW.tar 24.7 Mb (http)(custom) TAR (of TXT)

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