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Series GSE248172 Query DataSets for GSE248172
Status Public on Jan 10, 2024
Title Mitochondrial complex I activity in microglia sustains neuroinflammation [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sustained smouldering, or low grade, activation of myeloid cells is a common hallmark of several chronic neurological diseases, including multiple sclerosis (MS). Distinct metabolic and mitochondrial features guide the activation and the diverse functional states of myeloid cells. However, how these metabolic features act to perpetuate neuroinflammation is currently unknown. Using a multiomics approach, we identified a new molecular signature that perpetuates the activation of myeloid cells through mitochondrial complex I (CI) activity driving reverse electron transport (RET) and the production of reactive oxygen species (ROS). Blocking CI activity in pro-inflammatory microglia protected the central nervous system (CNS) against neurotoxic damage and improved functional outcomes in animal disease models in vivo. Our data show that mitochondrial CI in microglia is a potential new therapeutic target to foster neuroprotection in smouldering inflammatory CNS disorders.
 
Overall design MOG-EAE induction in Cx3cr1YFPCreERT2:R26tdTomato mice
 
Contributor(s) Peruzzotti-Jametti L, Willis C, Krzak G, Pirvan L, Mohorianu I, Pluchino S
Citation(s) 38480879
Submission date Nov 17, 2023
Last update date Apr 10, 2024
Contact name Irina Mohorianu
E-mail(s) data-submissions@stemcells.cam.ac.uk
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (13)
GSM7906797 Peak EAE infiltrating, biol rep 1
GSM7906798 Control resident, biol rep 1
GSM7906799 Peak EAE resident, biol rep 1
This SubSeries is part of SuperSeries:
GSE248175 Mitochondrial complex I activity in microglia sustains neuroinflammation
Relations
BioProject PRJNA1041864

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248172_matrix_counts.csv.gz 69.3 Mb (ftp)(http) CSV
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Raw data are available in SRA

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