|
| Status |
Public on Jan 10, 2024 |
| Title |
Mitochondrial complex I activity in microglia sustains neuroinflammation [scRNA-seq II] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sustained smouldering, or low grade, activation of myeloid cells is a common hallmark of several chronic neurological diseases, including multiple sclerosis (MS). Distinct metabolic and mitochondrial features guide the activation and the diverse functional states of myeloid cells. However, how these metabolic features act to perpetuate neuroinflammation is currently unknown. Using a multiomics approach, we identified a new molecular signature that perpetuates the activation of myeloid cells through mitochondrial complex I (CI) activity driving reverse electron transport (RET) and the production of reactive oxygen species (ROS). Blocking CI activity in pro-inflammatory microglia protected the central nervous system (CNS) against neurotoxic damage and improved functional outcomes in animal disease models in vivo. Our data show that mitochondrial CI in microglia is a potential new therapeutic target to foster neuroprotection in smouldering inflammatory CNS disorders.
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| |
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| Overall design |
MOG-EAE induction in WT and Nd6 mice
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| Contributor(s) |
Peruzzotti-Jametti L, Willis C, Krzak G, Pirvan L, Mohorianu I, Pluchino S |
| Citation(s) |
38480879 |
| |
| Submission date |
Nov 17, 2023 |
| Last update date |
Apr 10, 2024 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
|
| Street address |
Puddicombe Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE248175 |
Mitochondrial complex I activity in microglia sustains neuroinflammation |
|
| Relations |
| BioProject |
PRJNA1041867 |
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