 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2023 |
| Title |
Next Generation Sequencing and Quantitative Analysis of mRNAs in Caenorhabditis elegans fbxl-5 Mutant Animals |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The molecular mechanisms that govern the metabolic commitment to reproduction, which often occurs at the expense of the soma, remain poorly understood. We identified the C. elegans F-box protein FBXL-5 as a negative regulator of maternal provisioning of vitellogenin lipoproteins, which mediate the transfer of intestinal lipids to the germline. Mutations in fbxl-5 partially suppress the vitellogenesis defects observed in the heterochronic mutants lin-4 and lin-29, both of which ectopically express fbxl-5 at the adult developmental stage. FBXL-5 functions cell-autonomously to negatively regulate expression of the vitellogenin genes; and consistently, intestinal over-expression of FBXL-5 is sufficient to inhibit vitellogenesis, impair overall lipid homeostasis, and reduce lifespan. Our epistasis analyses suggest that fbxl-5 functions in concert with cul-6, a cullin gene, and the Skp1-related genes skr-3 and skr-5 to regulate vitellogenesis. Additionally, fbxl-5 acts genetically upstream of rict-1, which encodes the core mTORC2 protein Rictor, to govern vitellogenesis. Together, our results reveal an unexpected role for a SCF ubiquitin-ligase complex in controlling intestinal lipid homeostasis by tuning mTORC2 activity.
|
| |
|
| Overall design |
The mRNAs of wild-type, lin-29(n333), fbxl-5(rhd43), and rict-1(mg360) day 1 adult animals were profiled by high-throughput sequencing, in triplicate.
|
| |
|
| Contributor(s) |
Dowen RH |
| Citation(s) |
38946799 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM137985 |
Regulation of lipid homeostasis by proliferative signaling pathways |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Robert Houston Dowen |
|
| |
| Submission date |
Nov 24, 2023 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Robert Houston Dowen |
| E-mail(s) |
dowen@email.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Cell Biology and Physiology
|
| Lab |
133 N. Medical Drive
|
| Street address |
321 Fordham Hall, CB7100
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7100 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22765 |
Illumina HiSeq 4000 (Caenorhabditis elegans) |
|
| Samples (18)
|
| GSM7917835 |
MT333, biol rep 1 |
| GSM7917836 |
MT333, biol rep 2 |
| GSM7917837 |
MT333, biol rep 3 |
| GSM7917838 |
DLS316, biol rep 1 |
| GSM7917839 |
DLS316, biol rep 2 |
| GSM7917840 |
DLS316, biol rep 3 |
| GSM7917841 |
DLS490, biol rep 1 |
| GSM7917842 |
DLS490, biol rep 2 |
| GSM7917843 |
DLS490, biol rep 3 |
| GSM7917844 |
DLS491, biol rep 1 |
| GSM7917845 |
DLS491, biol rep 2 |
| GSM7917846 |
DLS491, biol rep 3 |
| GSM7917847 |
DLS492, biol rep 1 |
| GSM7917848 |
DLS492, biol rep 2 |
| GSM7917849 |
DLS492, biol rep 3 |
|
| Relations |
| BioProject |
PRJNA1044836 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248602_DESeq2_DEGs_DLS316_v_N2.txt.gz |
45.7 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_DLS490_v_N2.txt.gz |
159.6 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_DLS491_v_DLS490.txt.gz |
27.3 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_DLS491_v_N2.txt.gz |
119.8 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_DLS492_v_MT333.txt.gz |
65.3 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_DLS492_v_N2.txt.gz |
312.6 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_DEGs_MT333_v_N2.txt.gz |
337.4 Kb |
(ftp)(http) |
TXT |
| GSE248602_DESeq2_norm_gene_counts.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE248602_raw_gene_read_counts.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...