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Series GSE248680 Query DataSets for GSE248680
Status Public on Mar 04, 2024
Title Therapeutic application of circular RNA aptamers in a mouse model of psoriasis
Organisms Mus musculus; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Other
Summary Efforts to advance RNA aptamers as a novel therapeutic modality have been limited by their susceptibilty to degradation and immunogenicity. In a previous study, we demonstrated synthesized double-stranded circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated Protein Kinase R (PKR). Here, we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon alpha (IFNα)/dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.
 
Overall design Refer to individual Series
 
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Submission date Nov 27, 2023
Last update date Mar 06, 2024
Contact name Li Yang
E-mail(s) liyang_fudan@fudan.edu.cn
Organization name Fudan University
Department Institutes of Biological Sciences
Street address 131 Dong-An Road
City Shanghai
ZIP/Postal code 200032
Country China
 
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL26526 Illumina NovaSeq 6000 (synthetic construct)
Samples (40)
GSM7918642 WT mouse with 0 day IMQ treatment ,repeat1
GSM7918643 WT mouse with 0 day IMQ treatment ,repeat2
GSM7918644 WT mouse with 1 day IMQ treatment ,repeat1
This SuperSeries is composed of the following SubSeries:
GSE248678 Ribo– RNA-seq of psoriasis mouse model
GSE248679 circSHAPE-MaP of EPIC
GSE253346 Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5
Relations
BioProject PRJNA1045575

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE248680_RAW.tar 75.7 Mb (http)(custom) TAR (of TXT, XLS)
SRA Run SelectorHelp

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