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Series GSE248680 Query DataSets for GSE248680
Status Public on Mar 04, 2024
Title Therapeutic application of circular RNA aptamers in a mouse model of psoriasis
Organisms Mus musculus; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Summary Efforts to advance RNA aptamers as a novel therapeutic modality have been limited by their susceptibilty to degradation and immunogenicity. In a previous study, we demonstrated synthesized double-stranded circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated Protein Kinase R (PKR). Here, we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon alpha (IFNα)/dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.
Overall design Refer to individual Series
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Submission date Nov 27, 2023
Last update date Mar 06, 2024
Contact name Li Yang
Organization name Fudan University
Department Institutes of Biological Sciences
Street address 131 Dong-An Road
City Shanghai
ZIP/Postal code 200032
Country China
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL26526 Illumina NovaSeq 6000 (synthetic construct)
Samples (40)
GSM7918642 WT mouse with 0 day IMQ treatment ,repeat1
GSM7918643 WT mouse with 0 day IMQ treatment ,repeat2
GSM7918644 WT mouse with 1 day IMQ treatment ,repeat1
This SuperSeries is composed of the following SubSeries:
GSE248678 Ribo– RNA-seq of psoriasis mouse model
GSE248679 circSHAPE-MaP of EPIC
GSE253346 Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5
BioProject PRJNA1045575

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Supplementary file Size Download File type/resource
GSE248680_RAW.tar 75.7 Mb (http)(custom) TAR (of TXT, XLS)
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