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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 04, 2024 |
| Title |
Therapeutic application of circular RNA aptamers in a mouse model of psoriasis |
| Organisms |
Mus musculus; synthetic construct |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Efforts to advance RNA aptamers as a novel therapeutic modality have been limited by their susceptibilty to degradation and immunogenicity. In a previous study, we demonstrated synthesized double-stranded circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated Protein Kinase R (PKR). Here, we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon alpha (IFNα)/dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.
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| Overall design |
Refer to individual Series
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| Web link |
https://www.nature.com/articles/s41587-024-02204-4
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| Citation(s) |
38653797 |
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| Submission date |
Nov 27, 2023 |
| Last update date |
Jun 03, 2024 |
| Contact name |
Li Yang |
| E-mail(s) |
liyang_fudan@fudan.edu.cn
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| Organization name |
Fudan University
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| Department |
Institutes of Biological Sciences
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| Street address |
131 Dong-An Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL26526 |
Illumina NovaSeq 6000 (synthetic construct) |
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| Samples (40)
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| GSM7918642 |
WT mouse with 0 day IMQ treatment ,repeat1 |
| GSM7918643 |
WT mouse with 0 day IMQ treatment ,repeat2 |
| GSM7918644 |
WT mouse with 1 day IMQ treatment ,repeat1 |
| GSM7918645 |
WT mouse with 1 day IMQ treatment ,repeat2 |
| GSM7918646 |
WT mouse with 2 days IMQ treatment ,repeat1 |
| GSM7918647 |
WT mouse with 2 days IMQ treatment ,repeat2 |
| GSM7918648 |
WT mouse with 5 days IMQ treatment ,repeat1 |
| GSM7918649 |
WT mouse with 5 days IMQ treatment ,repeat2 |
| GSM7918650 |
PKR KO mouse with 0 day IMQ treatment ,repeat1 |
| GSM7918651 |
PKR KO mouse with 0 day IMQ treatment ,repeat2 |
| GSM7918652 |
PKR KO mouse with 1 day IMQ treatment ,repeat1 |
| GSM7918653 |
PKR KO mouse with 1 day IMQ treatment ,repeat2 |
| GSM7918654 |
PKR KO mouse with 2 days IMQ treatment ,repeat1 |
| GSM7918655 |
PKR KO mouse with 2 days IMQ treatment ,repeat2 |
| GSM7918656 |
PKR KO mouse with 5 days IMQ treatment ,repeat1 |
| GSM7918657 |
PKR KO mouse with 5 days IMQ treatment ,repeat2 |
| GSM7918658 |
EPIC-1 NAI,repeat 1 |
| GSM7918659 |
EPIC-1 NAI,repeat 2 |
| GSM7918660 |
EPIC-1 DC |
| GSM7918661 |
EPIC-1 DMSO |
| GSM7918662 |
EPIC-2 NAI,repeat1 |
| GSM7918663 |
EPIC-2 NAI,repeat2 |
| GSM7918664 |
EPIC-PKR NAI,repeat1 |
| GSM7918665 |
EPIC-PKR NAI,repeat2 |
| GSM7918666 |
EPIC-2 DC |
| GSM7918667 |
EPIC-2 DMSO |
| GSM7918668 |
EPIC-3 NAI,repeat1 |
| GSM7918669 |
EPIC-3 NAI,repeat2 |
| GSM7918670 |
EPIC-3 DC |
| GSM7918671 |
EPIC-3 DMSO |
| GSM7918672 |
EPIC-Cy5 NAI,repeat1 |
| GSM7918673 |
EPIC-Cy5 NAI,repeat2 |
| GSM7918674 |
EPIC-Cy5 DC |
| GSM7918675 |
EPIC-Cy5 DMSO |
| GSM7918676 |
EPIC-LNP NAI,repeat1 |
| GSM7918677 |
EPIC-LNP NAI,repeat2 |
| GSM7918678 |
EPIC-LNP DC |
| GSM7918679 |
EPIC-LNP DMSO |
| GSM8019042 |
IMQ mouse deliveried EPIC repeat1 |
| GSM8019043 |
IMQ mouse deliveried EPIC repeat2 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE248678 |
Ribo– RNA-seq of psoriasis mouse model |
| GSE248679 |
circSHAPE-MaP of EPIC |
| GSE253346 |
Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5 |
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| Relations |
| BioProject |
PRJNA1045575 |
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