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Series GSE24900 Query DataSets for GSE24900
Status Public on Oct 27, 2010
Title MicroRNA profiling analysis of differences between bone marrow mouse stem cells versus leukemic and teratocarcinomic cell lines
Organism Mus musculus
Experiment type Other
Summary Our aim is to identify any hallmark differentially expressed miRNAs between mouse bone marrow cells and different cancer cell lines (leukemic with lymphoid and myeloid origin and teratocarcinomic with dedifferentiated characteristics).
MicroRNAs (miRNAs) are an important member of posttrascriptional regulators and its pathological level changes have been implicated in various cancer types. This is the first step of the study focused at cancer stem cell biology of cancer in our in vivo mouse models.
We conclude by high-throughput microRNA profiling analysis,that exists a group of miRNAs candidates to explore their biological function in mice bone marrow stem cells an its role in carcinogenesis.
 
Overall design We isolated the bone marrow cells from mice by femur and tibial flush, then wash and collected. We isolate the same number of cells from fresh cultures of different cell lines types at passage 3, then wash and collected. Total RNA was extracted for the TaqMan Low Density Array v2.0.RNA quality was assessed using Nanodrop for concentration and purity (OD 260/280 and 260/230) and the Agilent Bioanalyzer 2100 for integrity (RNA integrity number (RIN) > 9).
Normalization was performed with common endogenous controls for A card and B card (MummU6, snoRNA135, snoRNA202 reference snRNAs for mouse).Comparative real-time PCR was performed in 3 replicates, including no-template controls.
 
Contributor(s) Martín-Fernández JM, González FJ
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Submission date Oct 25, 2010
Last update date Mar 22, 2012
Contact name J.M. Martin
Organization name OSP
Department CSC Biology
Street address Avd/Universidad de Coimbra s/n
City Salamanca
ZIP/Postal code 37008
Country Spain
 
Platforms (2)
GPL8526 Applied_Biosystems_Taqman_miRNA_Expression Assays_B (Mouse)
GPL8527 Applied_Biosystems_Taqman_miRNA_Expression Assays_A (Mouse)
Samples (24)
GSM612289 CT-BM R1
GSM612290 CT-BM R2
GSM612291 CT-BM R3
Relations
BioProject PRJNA132085

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24900_RAW_DATA.txt.gz 67.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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