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Series GSE24903 Query DataSets for GSE24903
Status Public on Dec 10, 2010
Title Splenic CD4 T cells in naïve C57BL/6 mice or during Plasmodium berghei ANKA infection in C57BL/6 and B6.GzmB-/- mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Infection of C57BL/6 mice with Plasmodium berghei ANKA (PbA) is a well-established experimental model of cerebral malaria (ECM). ECM is characterised by high levels of parasite sequestration and recruitment of pathogenic CD8+ T cells to the brain. The role of CD4+ T cells in this model has not yet been fully elucidated, although our laboratory has recently shown that CD4+ T cell depletion prior to infection results in significantly lower parasite burdens and protection from ECM. These data highlight a pathogenic role for CD4+ T cells in ECM.
Our unpublished observations indicate that mice lacking the CD8+ T cell cytolytic effector molecule, Granzyme B, (GzmB), are resistant to ECM, showing markedly reduced parasite burdens. Late depletion of CD4+ T cells from PbA-infected GzmB-deficient mice results in enhanced parasite burdens, indicating that these cells may play an anti-parasitic role. In the present study, we have analysed splenic CD4+ T cell gene expression profiles in naïve C57BL/6 mice, and PbA-infected wild-type and GzmB-deficent mice to further our understanding of the CD4+ T cell response in ECM.
Overall design Splenic CD4 T cells isolated by cell sorting from either uninfected C57BL/6 or PbA-infected C57BL/6 or B6.GzmB-/- mice at day 4 post-infection. Total RNA obtained from four mice per group.
Contributor(s) Desbarrieres L, Best SE, Boyle GM, Haque A, Engwerda CR
Citation(s) 21674481, 21525386
Submission date Oct 25, 2010
Last update date Jan 16, 2019
Contact name Ashraful Haque
Phone +61 7 33620414
Fax +61 7 38453507
Organization name Queensland Institute of Medical Research
Department Immunology
Lab Immunology and Infection
Street address 300 Herston Road
City Herston
State/province Brisbane
ZIP/Postal code QLD 4006
Country Australia
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (12)
GSM612349 CD4Tcells_GzmB-/-_PbA-infected_rep1
GSM612350 CD4Tcells_C57BL/6_PbA-infected_rep1
GSM612351 CD4Tcells_GzmB-/-_PbA-infected_rep2
BioProject PRJNA132091

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24903_RAW.tar 15.8 Mb (http)(custom) TAR
GSE24903_non-normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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