 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 03, 2023 |
| Title |
SERS analysis of cancer cell-secreted purines reveals a unique paracrine crosstalk in MTAP-deficient tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The tumor microenvironment (TME) is a dynamic pseudoorgan that shapes the development and progression of cancers. It is a complex ecosystem shaped by interactions between tumor and stromal cells. Although the traditional focus has been on the paracrine communication mediated by protein messengers, recent attention has turned to the metabolic secretome in tumors. Metabolic enzymes, together with exchanged substrates and products, have emerged as potential biomarkers and therapeutic targets. However, traditional techniques for profiling secreted metabolites in complex cellular contexts are limited. Surface-enhanced Raman scattering (SERS) has emerged as a promising alternative due to its nontargeted nature and simplicity of operation. Although SERS has demonstrated its potential for detecting metabolites in biological settings, its application in deciphering metabolic interactions within multicellular systems like the TME remains underexplored. In this study, we introduce a SERS-based strategy to investigate the secreted purine metabolites of tumor cells lacking methylthioadenosine phosphorylase (MTAP), a common genetic event associated with poor prognosis in various cancers. Our SERS analysis reveals that MTAP-deficient cancer cells selectively produce methylthioadenosine (MTA), which is taken up and metabolized by fibroblasts. Fibroblasts exposed to MTA exhibit: i) molecular reprogramming compatible with cancer aggressiveness, ii) a significant production of purine derivatives that could be readily recycled by cancer cells, and iii) the capacity to secrete purine derivatives that induce macrophage polarization. Our study supports the potential of SERS for cancer metabolism research and reveals an unprecedented paracrine crosstalk that explains TME reprogramming in MTAP-deleted cancers.
|
| |
|
| Overall design |
HBF in control conditions vs HBF treated with MTA to resemble the phenotype in MTAP -/- Tumors.
|
| |
|
| Contributor(s) |
Valera PS, Plou J, García I, Astobiza I, Viera C, Sasselli IR, Carracedo A, Liz-Marzán LM, Aransay AM, Martin JE |
| Citation(s) |
38109528 |
| |
| Submission date |
Nov 30, 2023 |
| Last update date |
Mar 05, 2024 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
|
| Phone |
0034944061325
|
| Organization name |
CIC bioGUNE
|
| Department |
Genome Analysis Platform
|
| Street address |
Parque tecnologico de Bizkaia, Building 801-A
|
| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
| GSM7926099 |
Control, Breast Fibroblasts, rep4 |
| GSM7926100 |
MTA Treated, Breast Fibroblasts, rep1 |
| GSM7926101 |
MTA Treated, Breast Fibroblasts, rep2 |
| GSM7926102 |
MTA Treated, Breast Fibroblasts, rep3 |
| GSM7926103 |
MTA Treated, Breast Fibroblasts, rep4 |
|
| Relations |
| BioProject |
PRJNA1047127 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE249082_AC-59_RawCounts.txt.gz |
5.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...