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Series GSE249507 Query DataSets for GSE249507
Status Public on Dec 07, 2023
Title Modifiers of genetic dominance at the Arabidopsis self-incompatibility locus retain proto-miRNA features and act through non-canonical gene silencing pathways - AGO-IP data
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Self-incompatibility (SI) in flowering plants is a common mechanism that prevents self-fertilization and promotes outcrossing. In Brassicaceae, the locus controlling SI is highly diverse and dozens of distinct alleles are organized in a complex dominance hierarchy: the gene controlling SI specificity in pollen always shows monoallelic expression in heterozygote individuals, and this is achieved through the action of sRNA precursors that resemble miRNAs, although the mechanism behind this remains elusive. Here, we engineered Arabidopsis thaliana lines expressing components of the Arabidopsis halleri SI system and used a reverse genetics approach to pinpoint the pathways underlying the function of these sRNA precursors. We showed that they trigger a robust decrease in transcript abundance of the recessive pollen SI genes, but not through the canonical transcriptional or post transcriptional gene silencing pathways. Furthermore, we observed that single sRNA precursors are typically processed into hundreds of sRNA molecules, with distinct sizes, abundance levels and ARGONAUTE loading preferences. This heterogeneity closely resembles that of proto-miRNAs, the evolutionary ancestors of miRNAs. Our results suggest that these apparent arbitrary features, which are often associated with lack of effects in gene expression, are crucial in the context of the SI dominance hierarchy since they allow one sRNA precursor of a given allele to repress multiple other (more recessive) alleles. This study not only provides an in-depth characterization of the molecular features underlying complex dominance interactions, but also constitutes a unique example of how specific evolutionary constraints shape the progression of sRNA precursors within the proto-miRNA – miRNA evolutionary continuum.
 
Overall design small RNA sequencing after ARGONAUTE Immunoprecipitation. AGO1- and AGO4-IPs were performed on immature floral buds of transgenic A. thaliana plants expressing the S locus sRNA precursors Ah04mir1887, Ah20mirS3 or Ah20mirS2 from A. halleri.
 
Contributor(s) A Batista R, Azevedo-Favory J, Lagrange T, Castric V
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Submission date Dec 06, 2023
Last update date Mar 20, 2024
Contact name Vincent Castric
E-mail(s) vincent.castric@univ-lille.fr
Organization name CNRS / Universite de Lille - Sciences et Technologies
Lab Unite Evo-Eco-Paleo (EEP) - UMR 8198
Street address Batiment SN2, bureau 207
City Villeneuve d'Ascq
ZIP/Postal code 59655
Country France
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (9)
GSM7949154 Ah04mir1887_Input
GSM7949155 Ah04mir1887_AGO1-IP
GSM7949156 Ah04mir1887_AGO4-IP
This SubSeries is part of SuperSeries:
GSE249508 Modifiers of genetic dominance at the Arabidopsis self-incompatibility locus retain proto-miRNA features and act through non-canonical gene silencing pathways
Relations
BioProject PRJNA1049412

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE249507_mir1887_sRNA_db.txt.gz 1.5 Kb (ftp)(http) TXT
GSE249507_mirS2_sRNA_db.txt.gz 1.1 Kb (ftp)(http) TXT
GSE249507_mirS3_sRNA_db.txt.gz 1.7 Kb (ftp)(http) TXT
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