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Series GSE249782 Query DataSets for GSE249782
Status Public on May 17, 2024
Title Characterization of age-associated gene expression changes in mouse sweat glands [FP_WT_TA]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Evaporation of sweat on the skin surface is the major mechanism for dissipating heat in humans. The secretory capacity of sweat glands (SWGs) declines during aging, leading to heat intolerance in the elderly, but the mechanisms responsible for this decline remain incompletely understood. We investigated the molecular changes accompanying SWG aging in mice, where sweat tests confirmed a significant reduction of active SWGs in old mice relative to young mice. We first identified SWG-enriched mRNAs by comparing the transcriptome of Eda mutant Tabby mice, which lack SWGs, with that of wild-type control mice by RNA-sequencing analysis. This comparison revealed 171 mRNAs enriched in SWGs, including 47 mRNAs encoding core secretory proteins such as transcription factors, ion channels, ion transporters, and trans-synaptic signaling proteins. Among these, 28 SWG-enriched mRNAs showed significantly altered abundance in the aged footpad skin, and 11 of them, including Foxa1, Best2, Chrm3, and Foxc1 mRNAs belonged to the ‘core secretory’ category. Consistent with the changes in mRNA expression, immunohistology revealed that higher numbers of secretory cells from old SWGs express FOXC1 protein, the product of Foxc1 mRNA. In sum, our study identified mRNAs enriched in SWGs, including those that encode core secretory proteins, and changes in these mRNAs and proteins with SWG aging in mice.
Overall design Footpad skin from fore paws of 3 months old Eda mutant Tabby male mice and age and sex matched C57BL/6J control mice was colllected under a dissection microscope. Three biological replicates for each genotype. One mouse = one biological replicate. Total RNA was isolated using Qiagen RNeasy Fibrous Tissue Mini Kit (Qiagen Cat# 74704). Sequencing library was prepared using Illumina TruSeq Stranded mRNA Library prep kit (Illumina Cat# 20020594). Libraries were subsequently sequenced for Paired-End sequencing with Illumina NovaSeq 6000 sequencer.
Contributor(s) Zonnefeld A, Cui C, Tsitsipatis D, Piao Y, Fan J, Mazan-Mamczarz K, Xue Y, Indig FE, De S, Gorospe M
Citation(s) 38637019
Submission date Dec 08, 2023
Last update date May 18, 2024
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7964192 WT_FP_1
GSM7964193 WT_FP_2
GSM7964194 WT_FP_3
This SubSeries is part of SuperSeries:
GSE249784 Characterization of age-associated gene expression changes in mouse sweat glands
BioProject PRJNA1050328

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE249782_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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