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Series GSE250137 Query DataSets for GSE250137
Status Public on Nov 08, 2024
Title METTL14-mediated m6A differentially orchestrates brown and white adipose tissue transcriptomes to regulate systemic metabolism
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Human brown and white adipocytes (hBAT and hWAT) display markedly distinct m6A landscapes; besides, in insulin-resistant humans and mice, methyltransferase like 14 (METTL14) expression differs significantly between BAT and WAT in the context of its correlation with insulin sensitivity. We therefore employed independent BAT- and WAT-specific METTL14 knockout models to unveil the cell-type specificity of m6A mRNA methylation. Mettl14 knockout via Ucp1-cre or Adipoq-cre drivers in BAT and WAT, respectively, yields divergent metabolic outcomes in mouse.
 
Overall design Mettl14fl/fl mice on a C57BL/6N background (De Jesus et al. 2019) were crossed with Ucp1cre strain (stock no.024670, the Jackson Laboratory) or Adipoqcre strain (stock no.028020, the Jackson Laboratory) mice, which do not harbor the nicotinamide nucleotide transhydrogenase (Nnt) mutation, to generate the knockouts.
For studies on diet-induced obesity and long-term insulin resistance, M14fl/fl,M14fl/fl-Ucp1cre, and M14fl/fl-Adipoqcre mice were fed either 10% fat LFD or a conventional 60% fat HFD for 14 weeks, starting at 6 weeks of age. To examine tissue-specific insulin sensitivity, mice were fasted for 16 hours.
 
Contributor(s) Ju C, Xiao L, Kulkarni RN, He C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 13, 2023
Last update date Nov 08, 2024
Contact name Cheng-Wei Ju
E-mail(s) cwju@uchicago.edu
Organization name The University of Chicago
Lab Chuan He Lab
Street address 929 East 57th Street, GCIS, Room E313
City Chicago
State/province Illinois
ZIP/Postal code 60637
Country USA
 
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (58)
GSM7974417 human brown adipocyte cell line, Control, input, biol rep 1
GSM7974418 human brown adipocyte cell line, Control, input, biol rep 2
GSM7974419 human brown adipocyte cell line, Control, input, biol rep 3
Relations
BioProject PRJNA1052092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE250137_M14.iBAT.Ucp1.Deseq2.csv.gz 2.5 Mb (ftp)(http) CSV
GSE250137_M14.iBAT.Ucp1.RADAR.csv.gz 488.2 Kb (ftp)(http) CSV
GSE250137_M14.iWAT.Adipoq.Deseq2.csv.gz 2.4 Mb (ftp)(http) CSV
GSE250137_M14.iWAT.Adipoq.RADAR.csv.gz 813.9 Kb (ftp)(http) CSV
GSE250137_hBAT.Deseq2.csv.gz 2.2 Mb (ftp)(http) CSV
GSE250137_hBAT.RADAR.csv.gz 45.3 Kb (ftp)(http) CSV
GSE250137_hWAT.Deseq2.csv.gz 2.6 Mb (ftp)(http) CSV
GSE250137_hWAT.RADAR.csv.gz 128.2 Kb (ftp)(http) CSV
GSE250137_hWAT.siKD.Deseq2.csv.gz 2.4 Mb (ftp)(http) CSV
GSE250137_hWAT.siKD.RADAR.csv.gz 85.3 Kb (ftp)(http) CSV
GSE250137_hWAT_hBAT.Deseq2.csv.gz 2.5 Mb (ftp)(http) CSV
GSE250137_hWAT_hBAT.RADAR.csv.gz 651.8 Kb (ftp)(http) CSV
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