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Series GSE25134 Query DataSets for GSE25134
Status Public on Dec 31, 2010
Title Expression data from dark-grown BR double mutant bzr1-1D;bri1-116, single mutant bri1-116, and wildtype
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Molecular genetic analyses support a central role of BZR1 in Brassinosteroid (BR) regulation of plant development. The dominant bzr1-1D mutation, which stabilizes the BZR1 protein, completely suppresses the de-etiolated phenotype of the null bri1-116 mutant grown in the dark.
Using microarray analysis, we identified genes differentially expressed in bri1-116 compared to wild type and genes differentially expressed in the bzr1-1D;bri1-116 double mutant compared to the bri1-116 single mutant. Consistent with the phenotypic suppression of bri1-116 by bzr1-1D, about 80% of the genes affected in bri1-116 were affected oppositely by bzr1-1D
Overall design BZR1 regulated genes were generated from comparing genes differentially expressed by bzr1-1D;bri1-116 and bri1-116. Genes affected by BRI1 were generated from comparing differentially expressed genes of bri1-116 and Col control.
ANOVA was used to find genes whose expression was different between bzr1-1D;bri1-116 and bri1-116 or between bri1-116 and Col samples [see Supplementary file below].
Contributor(s) Wang Z
Citation(s) 21074725
Submission date Nov 04, 2010
Last update date Jun 12, 2017
Contact name Yu Sun
Organization name Carnegie Institution of Washington
Department plant biology
Lab Zhiyong wang
Street address 260 panama street
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (9)
GSM617572 bzr1-1Dxbri1-116, rep 1
GSM617573 bzr1-1Dxbri1-116, rep 2
GSM617574 bzr1-1Dxbri1-116, rep 3
This SubSeries is part of SuperSeries:
GSE25135 Brassinosteroid (BR) plant hormone signaling
BioProject PRJNA142765

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25134_RAW.tar 19.7 Mb (http)(custom) TAR (of CEL)
GSE25134_anova_001_raw_above_15.txt.gz 686.0 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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