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| Status |
Public on Dec 31, 2010 |
| Title |
Expression data from dark-grown BR double mutant bzr1-1D;bri1-116, single mutant bri1-116, and wildtype |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Molecular genetic analyses support a central role of BZR1 in Brassinosteroid (BR) regulation of plant development. The dominant bzr1-1D mutation, which stabilizes the BZR1 protein, completely suppresses the de-etiolated phenotype of the null bri1-116 mutant grown in the dark.
Using microarray analysis, we identified genes differentially expressed in bri1-116 compared to wild type and genes differentially expressed in the bzr1-1D;bri1-116 double mutant compared to the bri1-116 single mutant. Consistent with the phenotypic suppression of bri1-116 by bzr1-1D, about 80% of the genes affected in bri1-116 were affected oppositely by bzr1-1D
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| Overall design |
BZR1 regulated genes were generated from comparing genes differentially expressed by bzr1-1D;bri1-116 and bri1-116. Genes affected by BRI1 were generated from comparing differentially expressed genes of bri1-116 and Col control.
ANOVA was used to find genes whose expression was different between bzr1-1D;bri1-116 and bri1-116 or between bri1-116 and Col samples [see Supplementary file below].
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| |
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| Contributor(s) |
Wang Z |
| Citation(s) |
21074725 |
| |
| Submission date |
Nov 04, 2010 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Yu Sun |
| E-mail(s) |
yusun05@gmail.com
|
| Organization name |
Carnegie Institution of Washington
|
| Department |
plant biology
|
| Lab |
Zhiyong wang
|
| Street address |
260 panama street
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE25135 |
Brassinosteroid (BR) plant hormone signaling |
|
| Relations |
| BioProject |
PRJNA142765 |
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