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Series GSE25148 Query DataSets for GSE25148
Status Public on Jan 12, 2011
Title Changes in gene expression in HEK-TLR2 cells in response to Helicobacter pylori lipopolysaccharide
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study set out to identify global changes in gene expression in human embryonic kidney (HEK) cells stably transfected with Toll-like receptor 2 (TLR2) over a 48 hour time-course, following stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori.
Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point.
Overall design Total RNA was harvested from HEK-TLR2 cells following treatment with LPS for 2, 4, 8, 24 and 48h or untreated cells at the same time-point. 2 independent experiments were carried out. RNA was labeled and hybridized to GeneChips to analyse changes in gene expression.
Contributor(s) Smith SM, Moran AP, Duggan SP, Kelleher DP
Citation(s) 21220698
Submission date Nov 04, 2010
Last update date Mar 25, 2019
Contact name Sinead Marian Smith
Organization name Trinity College Dublin
Lab Trinity Centre for Health Sciences
Street address St. James's Hospital
City Dublin 8
ZIP/Postal code D8
Country Ireland
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (20)
GSM618017 HEK-TLR2 2h-LPS rep1
GSM618018 HEK-TLR2 2h-LPS rep2
GSM618019 HEK-TLR2 2h+LPS rep1
This SubSeries is part of SuperSeries:
GSE25155 Tribbles 3 - a novel regulator of TLR2-mediated signaling in response to Helicobacter pylori lipopolysaccharide
BioProject PRJNA142773

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25148_RAW.tar 92.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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