 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 26, 2024 |
| Title |
scRNA-Seq: First Atlas and Cellular Landscape of Lacrimal Sac: Implications in Primary Acquired Nasolacrimal Duct Obstruction Pathogenesis. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The aim of the study is to describe the transcriptional changes of individual cellular components in the lacrimal sac in patients with primary acquired nasolacrimal duct obstruction (PANDO) and attempt to construct the first lacrimal sac cellular atlas to elucidate the potential mechanisms that may drive the disease pathogenesis.
Methods: Lacrimal sac samples were obtained intraoperatively during the endoscopic dacryocystorhinostomy (EnDCR) procedure from five patients. Single-cell RNA sequencing was performed to analyze each individual cell population including epithelial and immune cells during the early-inflammatory and late-inflammatory phases of the disease.
Results: Eleven cell types were identified among 25,791 cells. T cells and B cells were the cell populations with the greatest variation in cell numbers between the two phases and were, involved in immune response and epithelium migration-related pathways. The present study showed that epithelial cells highly expressed the genes of senescence-associated secretory phenotype (SASP) and were involved in influencing the inflammation, neutrophil chemotaxis, and migration during the late-inflammatory stage. Enhanced activity of CXCLs-CXCRs between the epithelial cells and neutrophils was noted by the cell-cell communication analysis and is suspected to play a role in inflammation by recruiting more neutrophils.
Conclusions: The study presents a comprehensive single-cell landscape of the lacrimal sac cells in different phases of PANDO. The contribution of T, B cells and epithelial cells to the inflammatory response, and construction of the intercellular signaling networks between the cells within the lacrimal sac has further enhanced the present understanding of the PANDO pathogenesis.
|
| |
|
| Overall design |
using BD Rhapsody system in March 2023
|
| |
|
| Contributor(s) |
Zhang W, Huang H, Liu X, Zhang L, Li L, Ding Y, Xiao Y, Ali MJ, Sun H, Xiao C |
| Citation(s) |
38551583 |
| |
| Submission date |
Dec 26, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Caiwen Xiao |
| E-mail(s) |
183184@shsmu.edu.cn
|
| Phone |
+8613341731355
|
| Organization name |
Shanghai Ninth People's Hospital
|
| Street address |
Zhizaoju Road
|
| City |
Shanghai |
| State/province |
-- Select State/Province -- |
| ZIP/Postal code |
200000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA1057236 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE252058_RAW.tar |
89.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...