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| Status |
Public on Jan 12, 2024 |
| Title |
Malaria parasite manipulates erythrocyte membranes for intracellular adaptation : RNA-seq |
| Organism |
Plasmodium berghei |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ability to respond to stressful host environments is crucial for the survival of intracellular pathogens, during which transporters play key roles in the nutrient scavenging, such as lipid uptake. Here we discover a conserved malarial gene that orchestrates the parasitic adaption in the terminally differentiated, phospholipid scarce erythrocytes. The expression level of this gene, here named CAP, is the intensively upregulated in the erythrocyte-infecting parasites than the reticulocyte-infecting counterparts. We show that CAP encodes a small protein exported onto the membrane of infected erythrocytes where it interacts with host CTL1, a choline and ethanolamine transporter, and upregulates biosynthesis of phosphatidylcholine and phosphatidylethanolamine, the major component of parasite membrane. Deletion of CAP causes Plasmodium to upturn reticulocyte invasion and sexual stage differentiation. This work presents a novel mechanism for intracellular parasite to import lipid precursors into parasitized cells by manipulating an endogenous host transporter.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for wild type versus CAP knockout-conditioned P. berghei trophozoite stage cells
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| Contributor(s) |
Lin J, Wei H, Li C, Yang Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 08, 2024 |
| Last update date |
Jan 12, 2024 |
| Contact name |
chen li |
| E-mail(s) |
chenli5679@126.com
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| Organization name |
Sichuan Univercity
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| Street address |
Renmin South Road
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| City |
Chengdu |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL20293 |
Illumina HiSeq 2500 (Plasmodium berghei) |
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| Samples (10)
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| GSM8005429 |
P. berghei ANKA trophozoite stage cells, wild type, replicate 1 |
| GSM8005430 |
P. berghei ANKA trophozoite stage cells, wild type, replicate 2 |
| GSM8005431 |
P. berghei ANKA trophozoite stage cells, wild type, replicate 3 |
| GSM8005432 |
P. berghei ANKA trophozoite stage cells, wild type, replicate 4 |
| GSM8005433 |
P. berghei ANKA trophozoite stage cells, wild type, replicate 5 |
| GSM8005434 |
P. berghei ANKA trophozoite stage cells, CAP knockout, replicate 1 |
| GSM8005435 |
P. berghei ANKA trophozoite stage cells, CAP knockout, replicate 2 |
| GSM8005436 |
P. berghei ANKA trophozoite stage cells, CAP knockout, replicate 3 |
| GSM8005437 |
P. berghei ANKA trophozoite stage cells, CAP knockout, replicate 4 |
| GSM8005438 |
P. berghei ANKA trophozoite stage cells, CAP knockout, replicate 5 |
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| Relations |
| BioProject |
PRJNA1062291 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE252704_RAW.tar |
430.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE252704_raw_counts.txt.gz |
98.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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