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Series GSE25291 Query DataSets for GSE25291
Status Public on Dec 10, 2010
Title Altered antisense-to-sense transcript ratios in breast cancer: Sage-Seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptome profiling studies suggest that a large fraction of the genome is transcribed and many transcripts function independent of their protein coding potential. The relevance of noncoding RNAs (ncRNAs) in normal physiological processes and in tumorigenesis is increasingly recognized. Here, we describe consistent and significant differences in the distribution of sense and antisense transcripts between normal and neoplastic breast tissues. Many of the differentially expressed antisense transcripts likely represent long ncRNAs. A subset of genes that mainly generate antisense transcripts in normal but not cancer cells is involved in essential metabolic processes. These findings suggest fundamental differences in global RNA regulation between normal and cancer cells that might play a role in tumorigenesis.
Overall design Global transcriptome profilings of 12 samples in normal and 9 samples in cancer from clinical breast tissue using Sage-Seq.
Contributor(s) Polyak K, Reo M, Shipitsin M, Choudhury S, Wu ZJ, Protopopov A, Yao J, Lo P, Bessarabova M, Ishkin A, Nikolsky Y, Liu XS, Sukumar S
Citation(s) 21098291
Submission date Nov 11, 2010
Last update date May 15, 2019
Contact name Kornelia Polyak
Phone 617-632-2106
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Lab Polyak
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (21)
GSM603077 epitheliumbreast-normal-N1
GSM603078 epitheliumbreast-normal-N2
GSM603079 epitheliumbreast-normal-N3
This SubSeries is part of SuperSeries:
GSE25292 Altered antisense-to-sense transcript ratios in breast cancer
SRA SRP004847
BioProject PRJNA142565

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Supplementary file Size Download File type/resource
GSE25291_RAW.tar 43.0 Mb (http)(custom) TAR (of TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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