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Series GSE253582 Query DataSets for GSE253582
Status Public on May 29, 2024
Title Transcriptional profiling of CD11b+ microglia isolated from cerebellum and olfactory bulb-removed brains of SAMP8 and SAMR1 mice at 2, 5, and 9 months of age.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary SAMP8 mice serve as an accelerated aging model characterized by inflammation and neurological memory deficits, in contrast to their control SAMR1 counterparts. To investigate the role of microglia in aging, we conducted a comprehensive analysis of microglial heterogeneity using CITE-seq. CD11b positive cells were extracted from cerebellum and olfactory bulb-removed brains at key time points: 2 months, 5 months, and 9 months of age.
 
Overall design At 2, 5, and 9 months of age, brains of SAMP8 and SAMR1 mice were prepared by removing the cerebellum and olfactory bulbs. The remaining brain tissue was then physically and enzymatically dissociated in presence of Actinomycin D and Brefeldin A. Following dissociation, CITE-seq antibodies (ADTs and HTOs) and CD11b-PE/Cy5 staining were used. The DAPI-CD11b+ cells were subsequently isolated using fluorescence-activated cell sorting (FACS).
 
Contributor(s) Gruel R, Bijnens B, Van Den Daele J, Thys S, Verstraelen P, Roels J, Mancuso R, Vandamme N, De Vos WH
Citation(s) 38403918
Submission date Jan 18, 2024
Last update date May 29, 2024
Contact name Jana Roels
E-mail(s) jana.roels@vib.be, niels.vandamme@vib.be
Organization name VIB
Department VIB Single cell core
Street address Technologiepark-Zwijnaarde 71
City Zwijnaarde
ZIP/Postal code 9052
Country Belgium
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM8023836 9-months SAMR1, GEX
GSM8023837 9-months SAMR1, ADT
GSM8023838 9-months SAMR1,HTO
Relations
BioProject PRJNA1066315

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE253582_RGR001_raw_feature_bc_matrix.h5 102.4 Mb (ftp)(http) H5
GSE253582_RGR002_raw_feature_bc_matrix.h5 128.7 Mb (ftp)(http) H5
GSE253582_RGR003_raw_feature_bc_matrix.h5 59.7 Mb (ftp)(http) H5
GSE253582_RGR004_raw_feature_bc_matrix.h5 74.1 Mb (ftp)(http) H5
GSE253582_RGR008_raw_feature_bc_matrix.h5 51.1 Mb (ftp)(http) H5
GSE253582_RGR009_raw_feature_bc_matrix.h5 95.4 Mb (ftp)(http) H5
GSE253582_SAMP8.rds.gz 1.5 Gb (ftp)(http) RDS
GSE253582_readme.txt 5.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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