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| Status |
Public on May 15, 2024 |
| Title |
Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function [TraPR small RNA-seq] |
| Organism |
Zea mays subsp. mays |
| Experiment type |
Other
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Reproductive phasiRNAs are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs–named premeiotic 24-nt phasiRNAs–have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether premeiotic 24-nt phasiRNAs are also present in maize and related species and begin to characterize their biogenesis and function, we performed a comparative transcriptome and degradome analysis of premeiotic and meiotic anthers from five maize inbred lines and three teosinte species/subspecies. Our data indicate that a substantial subset of the 24-nt phasiRNA loci in maize and teosinte are already highly expressed at premeiotic phase. The premeiotic 24-nt phasiRNAs are similar to meiotic 24-nt phasiRNAs in genomic origin and dependence on DCL5 for biogenesis, but premeiotic 24-nt phasiRNAs are unique in that they are likely (i) not triggered by microRNAs, (ii) not loaded by AGO18 proteins, and (iii) not capable of mediating cis-cleavage. In addition, we also observed a group of premeiotic 24-nt phasiRNAs in rice using previously published data. Together, our results indicate that the premeiotic 24-nt phasiRNAs constitute a unique class of reproductive phasiRNAs and are present more broadly in the grass lineage than previously known.
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| Overall design |
Small RNA and degradome profiling of anther materials from five maize inbred lines, a maize mutant, and three teosinte species or subspecies
Please note that the *mm in sample titles represents the length of the anther used for the corresponding sample (e.g. 0.4-mm anther for 'B73-0.4mm-rep1' sample), and that the 'ago18HM' and 'ago18HT' represents 'ago18 triple homozygous mutant' and 'ago18 triple heterozygote', respectively.
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| Contributor(s) |
Zhan J, Bélanger S, Lewis S, Teng C, McGregor M, Beric A, Schon M, Nodine M, Meyers BC |
| Citation(s) |
38739785 |
| |
| Submission date |
Jan 30, 2024 |
| Last update date |
Jun 12, 2024 |
| Contact name |
Junpeng Zhan |
| E-mail(s) |
zhan@mail.hzau.edu.cn
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| Organization name |
Huazhong Agricultural University
|
| Street address |
1 Shizishan Street, Hongshan District
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL34147 |
NextSeq 550 (Zea mays subsp. mays) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE254584 |
Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function. |
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| Relations |
| BioProject |
PRJNA1071086 |
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