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| Status |
Public on Jul 22, 2024 |
| Title |
iSB09 elicits ABA-responsive gene expression in Solanum lycopersicum seedlings |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To compare the genome-wide transcriptional effect of ABA and iSB09 in tomato plants, we performed RNA-seq analysis of mock-, 10 uM ABA- or 20 uM iSB09-treated plants. Differential gene expression analysis between mock- and ABA-treated or iSB09-treated seedlings was done with DESeq2 and genes with an absolute value of log2 fold change (log2FC) > 1 or (log2FC) < -1 and p-adjusted value (padj) < 0.05 were selected. iSB09 upregulated and downregulated genes represent a subset of the ABA-responsive genes, which reflects the activation of PYL1-like and PYL4-like ABA receptors in tomato seedlings.
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| Overall design |
S. lycopersicum seedlings (3 d.a.g.) were transferred from plates to flashes with 3 ml of liquid Murashige and Skoog (MS medium) for 8 days in a growth chamber at 22ºC under long-day conditions (16 h light/8 h dark). Before treatment, the medium was refreshed and supplemented with either 0.1% DMSO (mock) or 10 μM ABA or 20 µM iSB09. Samples were collected after 3 hours of incubation, and the experiment was conducted with three independent biological replicates.
We performed genome-wide expression profiling analysis using data obtained from RNA-seq of the three biological replicates
Comparative analysis of transcriptional profiles using RNA-seq data for mock, iSB09 or ABA treatments (WT genotype)
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| Contributor(s) |
Illescas-Miranda J, Forment J, Rodriguez PL |
| Citation |
https://doi.org/10.1016/j.envexpbot.2024.105847
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| Submission date |
Feb 21, 2024 |
| Last update date |
Jul 22, 2024 |
| Contact name |
Pedro L. Rodriguez |
| E-mail(s) |
prodriguez@ibmcp.upv.es
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| Organization name |
IBMCP
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| Street address |
C/ Ingeniero Fausto Elio, s/n
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| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46011 |
| Country |
Spain |
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| Platforms (1) |
| GPL28981 |
DNBSEQ-G400 (Solanum lycopersicum) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1078758 |
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