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Series GSE256274 Query DataSets for GSE256274
Status Public on Dec 20, 2024
Title microRNA control of stem cell reconstitution and growth in root regeneration
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plants display a remarkable regeneration capacity, which allows them to replace damaged or lost cells, tissues and organs, and thus recover from a broad spectrum of injuries. Even lost stem cells can be regenerated from non-stem cells after competence acquisition, highlighting the enormous plasticity of plant cells. However, the molecular mechanisms underlying this process are still poorly understood. In the root, the highly conserved microRNA miR396 and its targets, the GROWTH REGULATING FACTORs (GRFs), control the transition from stem cells to proliferative cells. miR396 promotes stem cell activity by repressing the GRFs, which in turn promote cell proliferation. Here we show that the miR396-GRF regulatory module guides stem cell reconstitution after root tip excision, playing a dual role: while miR396 promotes competence, the GRFs control regeneration speed. Moreover, plants with ectopic miR396 expression have defined stem cell niches before the excision, but do not reconstitute them afterwards, remaining in an open state despite continuing to grow. We propose that this phenomenon is caused by dispersed stem cell activity, which supports growth after root tip excision without reconstituting the organized stem cell niche typical of Arabidopsis roots.
 
Overall design Protoplasts from cut (5 days after cut) or uncut (control) roots, from wild-type and miR396-overexpressing plants, were isolated per quadruplicate, and scRNA-seq was performed.
 
Contributor(s) Baulies JL, Rodríguez RE, Lazzara FE, Liebsch D, Zhao X, Zeng J, Bald L, Schommer C, Lohmann JU, Palatnik JF
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Submission date Feb 21, 2024
Last update date Dec 21, 2024
Contact name Julia Lidia Baulies
E-mail(s) baulies@ibr-conicet.gov.ar
Organization name Instituto de Biología Molecular y Celular de Rosario
Lab RNA Biology
Street address Ocampo y Esmeralda s/n
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platforms (1)
GPL24270 NextSeq 550 (Arabidopsis thaliana)
Samples (16)
GSM8092411 WT, Control, Replicate 1
GSM8092412 WT, 5DAC, Replicate 1
GSM8092413 p35S:MIR396, Control, Replicate 1
Relations
BioProject PRJNA1078846

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Supplementary file Size Download File type/resource
GSE256274_RAW.tar 1.5 Gb (http)(custom) TAR (of RDATA)
SRA Run SelectorHelp
Raw data are available in SRA

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