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Series GSE256329 Query DataSets for GSE256329
Status Public on Feb 27, 2024
Title Claudin-7 knockout by gene targeting
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the claudin-7 function in vivo, we generated a claudin-7 knockout mouse model by gene targeting through homologous recombination. In this study, we report that Cln7-/- pups show Na+, Cl-, and K+ wasting and chronic dehydration phenotypes. The dramatic increase of aldosterone synthase mRNA level suggests that these mice suffer from mishandling of NaCl and fluid in the distal nephrons. Deletion of claudin-7 invokes several compensatory changes, such as increased renin, serum-glucocorticoid-induced kinase 1 (SGK1), epithelial Na+ channel (ENaCα), Na+-Cl- cotransporter (NCC), as well as aquaporin (AQP) 2 mRNA levels, demonstrating the cross talks between paracellular and transcellular ion transport pathways.
Overall design 8 mouse intestinal samples were analyzed with 4 wildtype (WT) and 4 claudin-7 knockout (KO). Both WT and KO contain 2 small and 2 large intestinal samples.
Contributor(s) Chen Y, Xing T
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Submission date Feb 22, 2024
Last update date Feb 27, 2024
Contact name YAN-HUA Chen
Organization name University of South Carolina
Department Chemistry and Biochemistry
Street address 631 Sumter Street
City Columbia
State/province SC
ZIP/Postal code 29208
Country USA
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (8)
GSM8093804 #2 WT small intestine
GSM8093805 #2 KO small intestine
GSM8093806 #2 WT large intestine
BioProject PRJNA1079203

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Supplementary file Size Download File type/resource
GSE256329_RAW.tar 97.2 Mb (http)(custom) TAR (of TXT)

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