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Series GSE256471 Query DataSets for GSE256471
Status Public on May 24, 2024
Title Up-regulation of cholesterol synthesis by lysosomal defects requires a functional mitochondrial respiratory chain
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To understand the common and opposite effects of mitochondrial and lysosomal perturbations on cellular signaling, we performed RNAseq in HeLa cells stably silenced for the mitochondrial respiratory chain subunit UQCRC1 (as a model of chronic mitochondrial respiratory chain deficiency), for lysosomal hydrolase acid alpla-glucosidase (GAA), or for lysosomal cathepsin B (CTSB). The controls were HeLa cells with scrambled shRNA.
Overall design Two lines of HeLa-scrambled, two lines of UQCRC1-kd (each with a different shRNA), two lines of GAA-kd (each a different shRNA) and two lines of CTSB-kd (each with a different shRNA).
Web link
Contributor(s) Yambire KF, Raimundo N
Citation(s) 38782205
Submission date Feb 23, 2024
Last update date May 24, 2024
Contact name Nuno Raimundo
Organization name Penn State College of Medicine
Department Cellular and Molecular Physiology
Lab C4769
Street address 500 University Dr, College Of Medicine, Room C4769
City Hershey
State/province PA
ZIP/Postal code 17033
Country USA
Platforms (1)
GPL15433 Illumina HiSeq 1000 (Homo sapiens)
Samples (10)
GSM8099001 HeLa scrambled A1
GSM8099002 HeLa scrambled A2
GSM8099003 HeLa scrambled B1
BioProject PRJNA1079748

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Supplementary file Size Download File type/resource
GSE256471_HeLa_Lyso_samples_-_all_data_normalized.xlsx 2.1 Mb (ftp)(http) XLSX
GSE256471_HeLa_mito_samples_-_all_data_normalized.xlsx 1.8 Mb (ftp)(http) XLSX
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