|
| Status |
Public on May 24, 2024 |
| Title |
Up-regulation of cholesterol synthesis by lysosomal defects requires a functional mitochondrial respiratory chain |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the common and opposite effects of mitochondrial and lysosomal perturbations on cellular signaling, we performed RNAseq in HeLa cells stably silenced for the mitochondrial respiratory chain subunit UQCRC1 (as a model of chronic mitochondrial respiratory chain deficiency), for lysosomal hydrolase acid alpla-glucosidase (GAA), or for lysosomal cathepsin B (CTSB). The controls were HeLa cells with scrambled shRNA.
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| |
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| Overall design |
Two lines of HeLa-scrambled, two lines of UQCRC1-kd (each with a different shRNA), two lines of GAA-kd (each a different shRNA) and two lines of CTSB-kd (each with a different shRNA).
|
| Web link |
https://www.sciencedirect.com/science/article/pii/S0021925824019045?via%3Dihub
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| |
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| Contributor(s) |
Yambire KF, Raimundo N |
| Citation(s) |
38782205 |
| |
| Submission date |
Feb 23, 2024 |
| Last update date |
May 24, 2024 |
| Contact name |
Nuno Raimundo |
| Organization name |
Penn State College of Medicine
|
| Department |
Cellular and Molecular Physiology
|
| Lab |
C4769
|
| Street address |
500 University Dr, College Of Medicine, Room C4769
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA1079748 |
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