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Series GSE25739 Query DataSets for GSE25739
Status Public on Dec 02, 2010
Title Changes in expression of small non-coding RNAs, including microRNAs, in response to stress in C. elegans
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary To investigate the contribution of small non-coding RNAs, including microRNAs, to stress response in C. elegans, we examined their expression changes using Illumina deep-sequencing technology. Stress conditions we examined include heat shock, Pseudomonas aeruginosa (PA-14) and L1 arrest.

For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
 
Overall design Total RNAs were purified from wild-type young adult animals that were exposed to each stress condition, and used for cDNA library preparation for small RNAs. As for the L1 arrest sample, first wild-type embryos were collected and cultured without a food source in M9 buffer for 48 hrs with rotation, and then RNAs purified from L1 animals in a developmentally arrested state were used for the library preparation. These cDNA libraries established were sequenced with Illumina Genome Analyzer II.
 
Contributor(s) Kato M, Slack FJ
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BioProject PRJNA168961
Submission date Dec 01, 2010
Last update date May 15, 2019
Contact name Frank J Slack
E-mail(s) frank.slack@yale.edu
Organization name Yale University
Department MCDB
Street address 266 Whitney Ave
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platforms (1)
GPL9269 Illumina Genome Analyzer II (Caenorhabditis elegans)
Samples (4)
GSM632210 Control_Day1_wt
GSM632211 Heatshock_Day1_wt
GSM632212 PA14_Day1_wt
Relations
SRA SRP004785

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25739_RAW.tar 23.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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