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Series GSE25761 Query DataSets for GSE25761
Status Public on Dec 01, 2010
Title SAGE analysis of maize root segments
Organism Zea mays
Experiment type Expression profiling by SAGE
Summary Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) water potential vermiculite. About 1,000 roots were collected. Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. Details of the conditions and nutrient modifications have been described before (Sharp et al., 1988; Spollen et al, 2000). From each segment, 250 mg of material was used and RNA was isolated form the combined sample containing all four segments. RNA was isolated using the RNeasy Maxi Kit (Qiagen). RNA integrity was verified by denaturing agarose gel electorphoresis and spectrophotometry.
Overall design This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.
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Submission date Dec 01, 2010
Last update date Mar 22, 2012
Contact name Mark Fredricksen
Phone (217)265-5473
Organization name University of Illinois
Department Plant Biology
Lab Bohnert Laboratory
Street address 1201 W Gregory Dr
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platforms (1)
GPL1244 SAGE:10:NlaIII:Zea mays
Samples (1)
GSM23443 ZMR Sage
BioProject PRJNA134057

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Supplementary data files not provided
Processed data included within Sample table

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