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Status |
Public on Dec 10, 2010 |
Title |
E0-12h_HDAC11-495 |
Project |
modENCODE
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Organism |
Drosophila melanogaster |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
modENCODE_submission_2567 This submission comes from a modENCODE project of Kevin White. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The White Lab is aiming to map the association of many of the non-histone chromosomal proteins on the genome of Drosophila melanogaster. One technique that we use for this purpose is chromatin immunoprecipitation-on-chip (ChIP-on-chip) utilizing whole-genome tiling arrays. The data generated by ChIP-chip experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested chromatin-binding protein. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf CHIP-chip
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Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] ); Developmental Stage: Embryo 0-12h; Genotype: y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1]; NUMBER OF REPLICATES: 6; EXPERIMENTAL FACTORS: Developmental Stage Embryo 0-12h; Antibody HDAC11-495 (target is HDAC11); Strain Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] )
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Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
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Contributor(s) |
White K, Negre N, Bild N |
Citation(s) |
21430782 |
BioProject |
PRJNA63467 |
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Submission date |
Dec 09, 2010 |
Last update date |
Jul 08, 2015 |
Contact name |
DCC modENCODE |
E-mail(s) |
help@modencode.org
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Phone |
416-673-8579
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Organization name |
Ontario Institute for Cancer Research
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Lab |
modENCODE DCC
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Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 0A3 |
Country |
Canada |
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Platforms (1) |
GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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Samples (6)
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GSM637728 |
E0-12h_HDAC11-495_1 extraction1_array1 |
GSM637729 |
E0-12h_HDAC11-495_2 extraction2_array1 |
GSM637730 |
E0-12h_HDAC11-495_3 extraction3_array1 |
GSM637731 |
E0-12h_HDAC11-495_4 extraction4_array1 |
GSM637732 |
E0-12h_HDAC11-495_5 extraction5_array1 |
GSM637733 |
E0-12h_HDAC11-495_6 extraction6_array1 |
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