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| Status |
Public on Mar 12, 2024 |
| Title |
Soma-to-Germline miRNA inheritance via Yolk Promotes Stress Resilience in Progeny [mRNA] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
At the onset of reproduction, oviparous animals synthesize large amounts of yolk in somatic tissues to provide lipids and other nutrients to their progeny. However, whether the yolk transports other types of molecules, such as RNAs with gene regulatory functions, remains largely unexplored. Here, we have biochemically purified the yolk in the nematode Caenorhabditis elegans and show it contains microRNAs (miRNAs). We provide evidence that the yolk transports such miRNAs from the mother’s intestine to the embryos via the lipoprotein yolk receptor RME-2. These yolk-enriched miRNAs inherited by the embryos regulate the transcriptomes of developing larvae. Moreover, environmental stresses and maternal age modulate the transfer of yolk-enriched miRNAs, providing stress resilience benefits to progeny. This discovery establishes a novel paradigm in intergenerational gene regulation, where the gut-germline axis orchestrates the transmission of environmental cues through yolk-enriched miRNAs. Our work reveals a new mechanism underlying the soma-to-germline transfer of epigenetic information in animals.
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| Overall design |
RNA sequencing profiles of 2-cell embryos or freshly hatched L1 larvae from drosha pasha intestinal degron strain.
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| Contributor(s) |
Aupérin N, Singh M, Bourdon L, Commere P, Chervova A, Lopez-Maestre H, Gkaraveli A, Cecere G |
| Citation missing |
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| |
| Submission date |
Mar 11, 2024 |
| Last update date |
Jul 09, 2024 |
| Contact name |
Germano Cecere |
| E-mail(s) |
germano.cecere@pasteur.fr
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| Phone |
0033140613225
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| Organization name |
Institut Pasteur
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| Department |
Development and stem cell biology
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| Lab |
Mechanisms of Epigenetic Inheritance
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| Street address |
Institut Pasteur, 28 Rue Du Docteur Roux, Batiment Monod, 4eme Etage
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| City |
Paris |
| ZIP/Postal code |
75724 |
| Country |
France |
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| Platforms (1) |
| GPL32326 |
NextSeq 2000 (Caenorhabditis elegans) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA1086515 |
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