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Series GSE26135 Query DataSets for GSE26135
Status Public on Dec 18, 2013
Title Gene analysis of flagellin-treated nasal mucosa of Duox2-/- mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary To evaluate the roles of DUOX2 in flagellin- induced inflammatory response in mouse nasal mucosa. Wild type (Duox2+/+) and Duox2 knockout (Duox2-/-) mice were stimulated with 1 μg/ml of flagellin for 4h.
422 genes (> 2 fold) were up-regulated in nasal mucosa of Duox2+/+ that was treated with flagellin and the full list of genes is presented in Supplemental Table II. These genes included the following defense- and immune response-related genes : Cytokine/chemokine-related genes (CCL20, CCR2, CCR5, CXCL2, CXCL5, CXCL9, CXCL16, IL18RAP, TNFAIP2, IL1B EAR2, FPR1), Granulocyte-related genes (IL8RB, MPO, PRG2, PPBP, PRG3), interferon-related genes (IFITM6, IFI47), macrophage related genes (IL1B, S100A9), and T-cell mediated immune response related genes (H2-Q6, IL1F9). In addition, signal transduction (PPBP, OLFR60, P2RY10) and cell adhesion (SELL, SELP, ICAM1, DSG1A, DSG3, VCAM1) related genes were also increased by flagellin treatment. These genes were selected based on the biological processes and molecular functions of their gene ontology. However, the increase of inflammation and immune response related genes by flagellin treatment were diminished in the nasal mucosa of the Duox2-/- mice compared with that of Duox2+/+ mice.
 
Overall design Wild type (Duox2+/+) and Duox2 knockout (Duox2-/-) mice received either PBS (control) or flagellin (5 ug/ml) intranasally for 4h.
 
Contributor(s) Joo J, Bae YS, Yoon J
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Submission date Dec 17, 2010
Last update date Mar 04, 2019
Contact name JUNGHEE JOO
E-mail(s) bioplan@yuhs.ac
Phone 82-2-2228-2667
Fax 82-2-2228-7998
Organization name Yonsei University
Department College of Medicine
Lab Research Center for Natural Human Defense System,
Street address 134 Shinchon-dong, Seodaemun-gu
City Seoul
ZIP/Postal code 120-752
Country South Korea
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (4)
GSM641801 PBS treated nasal mucosa of wild type mouse (C56BL/6)
GSM641802 Flagellin treated nasal mucosa of wild type mouse (C56BL/6)
GSM641803 PBS treated nasal mucosa of Duox2 knockout (Duox2-/-) mouse
Relations
BioProject PRJNA135383

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26135_RAW.tar 16.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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