GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE26136 Query DataSets for GSE26136
Status Public on Feb 01, 2011
Title Mammalian Dpy-30 regulates genomic H3K4 methylation and is essential for ES cell fate specification
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
Summary H3K4 methylation is associated with active genes and, along with H3K27 methylation, is part of a bivalent chromatin mark that typifies poised developmental genes in ESCs. However, its functional roles in ESC maintenance and differentiation are not established. Here we show that mammalian Dpy-30, a core subunit of SET1/MLL complexes, biochemically modulates H3K4 methylation in vitro, and directly regulates chromosomal H3K4me3 throughout the mammalian genome. Depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage. The differentiation defect is accompanied by defects in gene induction and H3K4 methylation at key developmental loci. Our results provide strong experimental evidence for the hypothesis that H3K4 methylation is an essential functional component of the bivalent mark during activation of developmental genes in ESCs.
Overall design Total RNAs from control or knockdown cells before and after RA-mediated differentiation were subjected to Illumina microarray analyses.

ChIP-enriched DNA from mouse ES cells was analyzed by Solexa sequencing.
Contributor(s) Jiang H, Roeder RG
Citation(s) 21335234
Submission date Dec 17, 2010
Last update date May 15, 2019
Contact name Hao Jiang
Organization name Rockefeller University
Lab Roeder Lab
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (3)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (10)
GSM641805 Non-hairpin shRNA, undifferentiated
GSM641806 Scramble shRNA, undifferentiated
GSM641807 RbBP5 shRNA, undifferentiated
SRA SRP006206
BioProject PRJNA135385

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26136_RAW.tar 1.3 Gb (http)(custom) TAR (of BED, TXT, WIG)
GSE26136_non-normalized.txt.gz 2.2 Mb (ftp)(http) TXT
GSE26136_normalized.txt.gz 2.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data included within Sample table
Processed data are available on Series record
Raw data are available in SRA
Raw data are available on Series record
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap