|
| Status |
Public on Mar 01, 2011 |
| Title |
Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [MSDK-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, and gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation.
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| |
|
| Overall design |
Global profiling of differentially methylated regions in 2 cell types from 6 individuals.
|
| |
|
| Contributor(s) |
Polyak K, Reo M, Choudhury S, Kowalczyk A, Haviv I, Beresford-Smith B, Conway T, Kaspi A, Merino V, Lo P, Sukumar S |
| Citation(s) |
21533021 |
| |
| Submission date |
Dec 17, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Kornelia Polyak |
| E-mail(s) |
kornelia_polyak@dfci.harvard.edu
|
| Phone |
617-632-2106
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Polyak
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (12)
|
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| This SubSeries is part of SuperSeries: |
| GSE26141 |
Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium |
|
| Relations |
| SRA |
SRP004934 |
| BioProject |
PRJNA142403 |
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