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Status |
Public on Apr 01, 2011 |
Title |
miRNA expression profiles of HBE cells at different stages |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
To identify miRNAs involved in ST-induced cell transformation, we used microarray chips containing 856 miRNA probes to define miRNA expression profiles in 16HBE,16HBER and 16HBERST cells.
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Overall design |
we created HBE cells at different stages of the cell transformation process (Pang et al., 2008). In brief, primary human bronchial epithelial cells were immortalized by expressing SV40 LT and hTERT (named as HBELH cells). HBER cells were created by introduction of an oncogenic version of H-Ras into HBELH cells. In agreement with previous studies, the expression of the oncogene H-Ras led to an increase in cell proliferation; however these cells (HBER) were unable to grow in soft agar or form tumors in immunodeficient mice. Subsequently, HBERST cells generated by the additional introduction of SV40 ST into HBER cells grew in an anchorage independent fashion and formed tumors in immunodeficient mice. Therefore, these cell lines represent distinct stages of immortalized cells (HBELH), pre-transformed cells (HBER) and transformed cells (HBERST). And then, the different miRNA expression profiles of HBE cells at different stages were assayed by miRNA microarray.
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Contributor(s) |
Chen W, Wang Q, Li D |
Citation(s) |
21460851 |
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Submission date |
Dec 18, 2010 |
Last update date |
Mar 22, 2012 |
Contact name |
chen wen |
E-mail(s) |
wenchen1107@163.com
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Organization name |
Sun Yat-sen University
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Department |
Department of Toxicology
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Street address |
74 Zhongshan Road 2
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City |
Guangzhou |
ZIP/Postal code |
510080 |
Country |
China |
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Platforms (1) |
GPL11332 |
μParaflo™ MicroRNA microarray Assay |
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Samples (2) |
GSM642432 |
miRNA expression profiles of L02 and 16HBE cell lines |
GSM642433 |
miRNA expression profiles of 16HBER and 16HBERST cell lines |
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Relations |
BioProject |
PRJNA135289 |
Supplementary file |
Size |
Download |
File type/resource |
GSE26166_RAW.tar |
1.3 Mb |
(http)(custom) |
TAR (of XLS) |
Processed data included within Sample table |
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