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| Status |
Public on Mar 27, 2024 |
| Title |
Chemically induced expansion of type 2 alveolar epithelial cell promotes regenerative lower airway repair |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To evaluate the effect of DPP4 inhibitor on regenerative repair of the lung upon injury, we carried out a single cell RNA sequencing study. In this study, orally administered sitagliptin was tested in LPS-induced lung injury model, followed by longitudinal single cell transcriptomic analysis with lung cells harvested at indicated time points.
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| Overall design |
We evaluated the effects of DPP4 inhibitors on AEC2 expansion by RNA sequencing and single cell RNA sequencing methods. An RNA sequencing experiment was performed to characterize the human small airway epithelial cells. Then we compared the transcriptomes of human small airway epithelial cells treated with DMSO, IGF1, sitagliptin, saxagliptin, NVP-728 and TGFβ1. To gain further insights into the effects of DPP4 inhibitor on lung damage repair, orally administered sitagliptin was tested in LPS-induced lung injury model, followed by longitudinal single cell transcriptomic analysis with lung cells harvested at indicated time points.
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| Contributor(s) |
Shao S, Zhang N, Specht GP, You S, Li S, Nguyen-Tran V, Joesph SB, Chatterjee AK, Chen JJ, Schultz PG, Bollong MJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 25, 2024 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Sida Shao |
| E-mail(s) |
sidashao90@outlook.com
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| Phone |
+8613706504419
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| Organization name |
Westlake University
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| Lab |
Sida Shao Lab
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| Street address |
18 Shilongshan Rd, Xihu District
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310024 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (11)
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| GSM8164403 |
non-treated, biological replicate 1, scRNAseq |
| GSM8164404 |
non-treated, biological replicate 2, scRNAseq |
| GSM8164405 |
non-treated, biological replicate 3, scRNAseq |
| GSM8164406 |
LPS induced, vehicle treated, day 2, biological replicate 1-4, scRNAseq |
| GSM8164407 |
LPS induced, sitagliptin treated, day 2, biological replicate 1-4, scRNAseq |
| GSM8164408 |
LPS induced, vehicle treated, day 3, biological replicate 1-4, scRNAseq |
| GSM8164409 |
LPS induced, sitagliptin treated, day 3, biological replicate 1-4, scRNAseq |
| GSM8164410 |
LPS induced, vehicle treated, day 4, biological replicate 1-4, scRNAseq |
| GSM8164411 |
LPS induced, sitagliptin treated, day 4, biological replicate 1-4, scRNAseq |
| GSM8164412 |
LPS induced, vehicle treated, day 8, biological replicate 1-4, scRNAseq |
| GSM8164413 |
LPS induced, sitagliptin treated, day 8, biological replicate 1-4, scRNAseq |
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| Relations |
| BioProject |
PRJNA1091645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262393_RAW.tar |
3.9 Gb |
(http)(custom) |
TAR (of LOOM, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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