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| Status |
Public on Apr 14, 2024 |
| Title |
Gene expression changes in primary human endometrial stromal cells following METTL3 knockdown during in vitro decidualization |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We developed an in vitro model in which primary human endometrial stromal cells (HESCs) were induced to differentiate through treatment with MPA and 8-Br-cAMP. SiRNA-mediated knockdown of METTL3 was performed on HESCs, 6 h prior to treatment of 8-Br-cAMP and MPA.
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| Overall design |
To investigate the effect of METTL3 on decidualization, HESCs were subjected to decidualization for 4 days after transfection of siRNA targeting METTL3 (RiboBio, Guangzhou, China; #siG151008053209-1-5; siN0000001-1-5 serving as negtive control) for 6 hours.
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| Contributor(s) |
Liu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 05, 2024 |
| Last update date |
Apr 15, 2024 |
| Contact name |
Ji-Long Liu |
| E-mail(s) |
jilongliu@scau.edu.cn
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| Organization name |
South China Agricultural University
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| Street address |
483 Wushan Rd
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| City |
Guangzhou |
| ZIP/Postal code |
510642 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1096725 |
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