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| Status |
Public on Jul 12, 2024 |
| Title |
IFN-γ activates an immune-like regulatory network in the cardiac vascular endothelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The regulatory mechanisms underlying the response to pro-inflammatory cytokines during myocarditis are poorly understood. Here, we use iPSC-derived cardiovascular progenitor cells (CVPCs) to model the response to interferon gamma (IFN-γ) during myocarditis. We generate RNA-seq and ATAC-seq for four CVPCs that were treated with IFN-γ and compare them with paired untreated controls. Transcriptional differences after treatment show that IFN-γ initiates an innate immune cell-like response in the vascular cardiac endothelium. IFN-γ treatment also shifts the CVPC transcriptome towards the adult coronary artery and aorta-like profile and expands the relative endothelial cell population in all four CVPC lines. Analysis of the accessible chromatin shows that IFN-γ is a potent chromatin remodeler and establishes an IRF-STAT immune-cell like regulatory network. Our findings reveal insights into the endothelial-specific protective mechanisms during myocarditis.
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| Overall design |
RNA-seq and ATAC-seq was generated for four iPSC-derived cardiovascular progenitor cells (CVPCs) stimulated with 100nM IFNg. Both stimulated and paired control CVPCs have molecular data resulting in 16 FASTQ files.
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| Contributor(s) |
Timothy D A, Kelly A F, Matteo D |
| Citation missing |
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| |
| Submission date |
Apr 09, 2024 |
| Last update date |
Jul 13, 2024 |
| Contact name |
Timothy D Arthur |
| E-mail(s) |
tdarthur40@gmail.com
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| Organization name |
UCSD
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| Department |
Pediatrics
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| Street address |
9500 Gilman Drive #0761
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0761 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1098322 |
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