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| Status |
Public on Apr 16, 2024 |
| Title |
Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Primary microRNA (pri-miRNA) transcripts are processed by a protein complex called the Microprocessor comprised of the ribonuclease Drosha and its RNA binding partner DGCR8/Pasha. We sequenced small RNAs from animals containing a mutation in the WW domain of C. elegans pash-1. We found that these mutants have a modest but widespread reduction in miRNA levels when grown at 20˚C, which is further enhanced when grown at 25˚C. The results demonstrate a requirement for the WW domain in processing miRNAs in C. elegans.
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| Overall design |
Small RNA high-throughput sequencing was done using RNA isolated from wild type and pash-1(ram33)mutant whole gravid adult C. elegans grown at 20˚C or 25˚C. The tinyRNA pipeline was used to quantitatively assess miRNA levels.
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| Contributor(s) |
Montgomery TA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 13, 2024 |
| Last update date |
Apr 16, 2024 |
| Contact name |
Taiowa A Montgomery |
| E-mail(s) |
tai.montgomery@colostate.edu
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| Organization name |
Colorado State University
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| Department |
Department of Biology
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| Street address |
1878 Campus Delivery
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| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80523 |
| Country |
USA |
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| Platforms (1) |
| GPL25147 |
HiSeq X Ten (Caenorhabditis elegans) |
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| Samples (14)
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| GSM8207446 |
pash-1(ram33), 20C, rep 1 |
| GSM8207447 |
pash-1(ram33), 20C, rep 2 |
| GSM8207448 |
pash-1(ram33), 20C, rep 3 |
| GSM8207449 |
wild type, 25C, rep 1 |
| GSM8207450 |
wild type, 25C, rep 2 |
| GSM8207451 |
wild type, 25C, rep 3 |
| GSM8207452 |
wild type, 25C, rep 4 |
| GSM8207453 |
pash-1(ram33), 25C, rep 1 |
| GSM8207454 |
pash-1(ram33), 25C, rep 2 |
| GSM8207455 |
pash-1(ram33), 25C, rep 3 |
| GSM8207456 |
pash-1(ram33), 25C, rep 4 |
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| Relations |
| BioProject |
PRJNA1099906 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE263914_wt_pash-1_20C_rpm_normalized_counts.csv.gz |
404.2 Kb |
(ftp)(http) |
CSV |
| GSE263914_wt_pash-1_25C_rpm_normalized_counts.csv.gz |
458.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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