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Series GSE26436 Query DataSets for GSE26436
Status Public on Jan 05, 2011
Title LIF-Dependent, Pluripotent Stem Cells Established from Inner Cell Mass of Porcine Embryos
Organism Sus scrofa
Experiment type Genome variation profiling by SNP array
SNP genotyping by SNP array
Summary The pig is important for agriculture and as an animal model in human and veterinary medicine, yet, despite over 20 years of effort, it has proved a difficult species from which to generate pluripotent stem cells analogous to those derived from mouse embryos. Here we report the production of LIF-dependent, so called naïve type, pluripotent stem cells from the inner cell mass of porcine blastocysts by up-regulating expression of KLF4 and POU5F1. These cells resemble mouse ES cells and are distinct from the FGF2-dependent, induced pluripotent cell type derived from porcine somatic cells.
High throughput SNP chip genotyping was conducted on Illumina's Porcine SNP60 BeadChip (WG-410, a service provided by Geneseek, NE, http://www.neogen.com/GeneSeek/). The results exhibited that the two lines pluripotent stem cells from the inner cell mass of porcine blastocysts were porcine origin and genetically distinct.
 
Overall design Porcine pluripotent stem cells were derived from the inner cell mass with transduction with human KLF4 by lentiviral transduction.
 
Contributor(s) Telugu BP, Roberts RM
Citation(s) 21705331
Submission date Jan 04, 2011
Last update date Mar 23, 2012
Contact name Bhanu Telugu
E-mail(s) telugub@missouri.edu
Phone 573-884-9601
Fax 573-884-9676
Organization name University of Missouri
Department Animal Sciences
Street address 1201 E.Rollins St
City Columbia
State/province MO
ZIP/Postal code 65211
Country USA
 
Platforms (1)
GPL11379 Illumina Porcine SNP60 BeadChip (WG-410)
Samples (2)
GSM648531 pESK I
GSM648532 pESK II
Relations
BioProject PRJNA136815

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26436_raw_data.txt.gz 1.3 Mb (ftp)(http) TXT
GSE26436_summary.xls.gz 7.0 Mb (ftp)(http) XLS
Processed data included within Sample table

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