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Status |
Public on Aug 04, 2011 |
Title |
Expression data from Tcf1-expressing Thy1+CD25+ T lineage cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
We used microarrays to perform a global gene expression analysis in Tcf1-expressing Thy1+CD25+ T lineage cells that develop on OP9 stroma in the absence of Notch1 signals. We compare this to the starting population, LMPP progenitors, and to control expressing T lineage cells that developed on OP9 stroma expressing Notch ligand DL4. The overall goal of this study was to determine if Tcf1 initiates T lineage specification in lymphoid progenitors. We found that Tcf1 was sufficient to upregulate many T lineage genes as compared to control expressing progenitors on OP9-DL4. Abstract of manuscript: The thymus imposes the T cell fate on incoming multipotent progenitors, but the molecular mechanisms are poorly understood. We show that transcription factor Tcf1 initiates T-lineage-specific gene expression. Tcf1 is downstream of Notch1 signaling and expressed in early T-cell progenitors. Progenitors deficient for Tcf1 are unable to initiate normal T-lineage specification. Conversely, ectopic expression of Tcf1 in hematopoietic progenitors is sufficient to induce expression of T-lineage specific genes in vitro. Thus, our study identifies Tcf1 as critically involved in the establishment T cell identity.
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Overall design |
Wiltype LMPPs were isolated by a FACSAria cell sorter and retrovirally transduced with a Tcf1-containing (Tcf1-VEX) or control vector (VEX) retrovirus. Tcf1-expressing cells and control-vector expressing cells were then seeded on OP9 stroma or OP9 stroma expressing Notch ligand DL4, respectively. On day 10, Thy1+CD25+ T lineage cells were sorted from Tcf1-expressing cells on OP9 stroma and compared to sorted LMPPs and Thy1+CD25+ T lineage cells that developed from control-vector expressing cells on OP9-DL4.
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Contributor(s) |
Brittany W, Anthony C, Alex C, Yumi Y, Avinash B |
Citation(s) |
21814277 |
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Submission date |
Jan 11, 2011 |
Last update date |
Mar 04, 2019 |
Contact name |
Avinash Bhandoola |
E-mail(s) |
bhandooa@mail.med.upenn.edu
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Phone |
2155730274
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Organization name |
University of Pennsylvania
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Department |
Pathology
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Lab |
266 John Morgan
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Street address |
3620 Hamilton Walk
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City |
Philadelphia |
State/province |
Philadelphia |
ZIP/Postal code |
19104 |
Country |
USA |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (4)
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GSM652995 |
Pooled wiltype LMPPs (Lineage marker-Sca+kit+Flt3high) |
GSM652996 |
Tcf1-expressing Thy1+CD25+ Cells Biological replicate #1 |
GSM652997 |
Tcf1-expressing Thy1+CD25+ Cells Biological replicate #2 |
GSM652998 |
Control vector expressing-Thy1+CD25+ Cells |
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Relations |
BioProject |
PRJNA136225 |