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Series GSE265909 Query DataSets for GSE265909
Status Public on May 15, 2024
Title yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution. [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Index sorted SMART-Seq2 sequencing to map AThi oHSC vs ATlo oHSC based on GFP-LC3 marker levels. Between yHSC and oHSC the largest transcriptional differences observed in the G0/G1 cell cycle phase cluster. Within oHSCs, AThi oHSCs were almost exclusively observed in the G0/G1 cluster, whereas ATlo oHSCs were spread across the activation continuum, with cells still in the G0/G1 cluster found more proximal to S cluster cells.
 
Overall design 96 HSCs were sorted into individual wells recording information on surface markers and GFP-LC3 level.
 
Contributor(s) Dellorusso P, Passegue E, Jose Calero Nieto F, Wang X
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 25, 2024
Last update date May 15, 2024
Contact name Paul Vincent Dellorusso
E-mail(s) paul.dellorusso@gmail.com
Phone 5166552491
Organization name Columbia University Irving Medical Center
Department Genetics and Development
Lab Passegue Lab
Street address 701 West 168th Street, 724 Hammer Health Sciences Center
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (563)
GSM8231288 GFP-LC3 yHSC_1
GSM8231289 GFP-LC3 oHSC_LO_1
GSM8231290 GFP-LC3 oHSC_MID_1
This SubSeries is part of SuperSeries:
GSE229137 Autophagy engaging and non-engaging hematopoietic stem cells
Relations
BioProject PRJNA1104666

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE265909_fcounts1.txt.gz 3.3 Mb (ftp)(http) TXT
GSE265909_fcounts2.txt.gz 3.4 Mb (ftp)(http) TXT
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Raw data are available in SRA

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