|
| Status |
Public on May 15, 2024 |
| Title |
yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution. [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Index sorted SMART-Seq2 sequencing to map AThi oHSC vs ATlo oHSC based on GFP-LC3 marker levels. Between yHSC and oHSC the largest transcriptional differences observed in the G0/G1 cell cycle phase cluster. Within oHSCs, AThi oHSCs were almost exclusively observed in the G0/G1 cluster, whereas ATlo oHSCs were spread across the activation continuum, with cells still in the G0/G1 cluster found more proximal to S cluster cells.
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| |
|
| Overall design |
96 HSCs were sorted into individual wells recording information on surface markers and GFP-LC3 level.
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| |
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| Contributor(s) |
Dellorusso P, Passegue E, Jose Calero Nieto F, Wang X |
| Citation(s) |
38754428 |
| |
| Submission date |
Apr 25, 2024 |
| Last update date |
Aug 15, 2024 |
| Contact name |
Paul Vincent Dellorusso |
| E-mail(s) |
paul.dellorusso@gmail.com
|
| Phone |
5166552491
|
| Organization name |
Columbia University Irving Medical Center
|
| Department |
Genetics and Development
|
| Lab |
Passegue Lab
|
| Street address |
701 West 168th Street, 724 Hammer Health Sciences Center
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (563)
|
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| This SubSeries is part of SuperSeries: |
| GSE229137 |
Autophagy engaging and non-engaging hematopoietic stem cells |
|
| Relations |
| BioProject |
PRJNA1104666 |
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