|
| Status |
Public on May 06, 2024 |
| Title |
ENL reads histone β-hydroxybutyrylation to modulate gene transcription (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Histone modifications are typically recognized by chromatin-binding protein modules (referred to as “readers”) to mediate fundamental processes such as transcription. Lysine β-hydroxybutyrylation (Kbhb) is a new type of histone mark that couples metabolism to gene expression. However, the readers that prefer histone Kbhb remain elusive. This knowledge gap must be filled in order to reveal the molecular mechanism of this epigenetic regulation. Herein, we developed a chemical proteomic approach, relying upon multivalent photoaffinity probes to capture binders of the mark and identified ENL as a novel target of H3K9bhb. Biochemical studies and CUT&Tag analysis further suggested that ENL favorably binds to H3K9bhb, and co-localizes with it on promoter regions to modulate gene expression. Notably, disrupting the interaction between H3K9bhb and ENL via structure-based mutation leads to the suppressed expression of the gene like MYC that drives cell proliferation.
|
| |
|
| Overall design |
we performed RNA-Seq by ectopically expressing WT-ENL or Mutant-ENL in cells
|
| |
|
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 30, 2024 |
| Last update date |
May 06, 2024 |
| Contact name |
爱源 王 |
| E-mail(s) |
way2022@tmu.edu.cn
|
| Phone |
15033260249
|
| Organization name |
TianjinMedical University
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics
|
| Street address |
No. 22, Meteorological Observatory Road, Heping District, Tianjin
|
| City |
Tianjin |
| State/province |
—Please choose an option— |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1106487 |
