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Series GSE266491 Query DataSets for GSE266491
Status Public on May 07, 2024
Title ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genes (PRO-seq)
Organism Homo sapiens
Experiment type Other
Summary Transcription factors bind to sequence motifs and act as activators or repressors. Transcription factors interface with a constellation of accessory cofactors to regulate distinct mechanistic steps to regulate transcription. We rapidly degraded the essential and ubiquitously expressed transcription factor ZNF143 to determine its function in the transcription cycle. ZNF143 facilitates RNA Polymerase initiation and activates gene expression. ZNF143 binds the promoter of nearly all its activated target genes. Paradoxically, ZNF143 binds near the site of genic transcription initiation to directly repress a subset of genes. Although ZNF143 stimulates initiation at ZNF143-repressed genes (i.e. those that increase expression upon ZNF143 depletion), the molecular context of binding leads to cis repression. ZNF143 competes with other more efficient activators for promoter access, physically occludes transcription initiation sites, and acts as a molecular roadblock to RNA Polymerase to repress genes in cis. The term "context specific" is often invoked to describe transcription factors that have both activation and repression functions. Here, we define the context and molecular mechanisms of ZNF143-mediated cis activation and repression.
 
Overall design HEK293T cells with ZNF143 tagged with an inducible degron tag were treated with drugs to induce ZNF143 degradation for 30 minutes, then collected for PRO-seq.
 
Contributor(s) Dong J, Guertin MJ
Citation(s) 38798607
Submission date May 02, 2024
Last update date Jun 06, 2024
Contact name Jinhong Dong
E-mail(s) jdong@uchc.edu
Organization name University of Connecticut
Department Center for Cell Analysis and Modeling
Street address 400 Farmington Ave
City Farmington
State/province CT
ZIP/Postal code 06030
Country USA
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (8)
GSM8248130 HEK_CloneZD29_30min_control_PRO-seq rep1
GSM8248131 HEK_CloneZD29_30min_control_PRO-seq rep2
GSM8248132 HEK_CloneZD29_30min_control_PRO-seq rep3
Relations
BioProject PRJNA1107334

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Supplementary file Size Download File type/resource
GSE266491_HEK_CloneZD29_30min_control_minus_PE1_scaled.bigWig 175.2 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_control_minus_PE2_scaled.bigWig 174.9 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_control_plus_PE1_scaled.bigWig 181.8 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_control_plus_PE2_scaled.bigWig 168.9 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_dTAGV1_minus_PE1_scaled.bigWig 173.5 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_dTAGV1_minus_PE2_scaled.bigWig 168.2 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_dTAGV1_plus_PE1_scaled.bigWig 230.8 Mb (ftp)(http) BIGWIG
GSE266491_HEK_CloneZD29_30min_dTAGV1_plus_PE2_scaled.bigWig 162.2 Mb (ftp)(http) BIGWIG
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