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Series GSE26669 Query DataSets for GSE26669
Status Public on Feb 03, 2011
Title Expression data of alloantigen stimulated splenocytes treated with leukocyte costimulatory blockade antibodies or no treatment
Organism Mus musculus
Experiment type Expression profiling by array
Summary To elucidate the gene expression “footprint” of antigenically challenged T-cells which had been treated with anti-LFA-1, CTLA4Ig, anti-CD40-ligand antibodies, we performed microarray gene expression analysis comparing the expression profile of costimulatory blockade treated and untreated responder T-cells.
Overall design Allogeneic murine splenocytes were co-cultured in an vitro set-up for a mixed lymphocyte reaction. For the experimental/treated group, the leuckocyte costimulatory blocking antibodies anti-LFA-1, CTLA4Ig, anti-CD40-ligand were added to the media, the control group did not have any antiodies added to the media. After 5 days of incubation the responder CD4 and CD8 T cells were isolated by FACS and the gene expression was analyzed using affymetrix microarray platforms.
Contributor(s) Pearl JI, Ghosh Z
Citation(s) 21362570
Submission date Jan 18, 2011
Last update date Feb 11, 2019
Contact name Kitchener D. Wilson
Organization name Stanford University
Street address S140 Grant Bldg
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM656409 CD4 T-Cell Costimulatory blockade treated #1
GSM656410 CD4 T-Cell Costimulatory blockade treated #2
GSM656411 CD4 T-Cell no treatment #1
BioProject PRJNA136415

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26669_RAW.tar 26.9 Mb (http)(custom) TAR (of CEL)
GSE26669_t-test.txt.gz 27.0 Kb (ftp)(http) TXT
Processed data are available on Series record
Processed data included within Sample table

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