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| Status |
Public on May 14, 2024 |
| Title |
Developmentally regulated alternate 3’ end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine the extent of alternative 3' end cleavage during Drosophila spermatogenesis, we performed 3' end sequencing of Drosophila testes from flies at different time points post heat shock (pHS) that were mutant for bam and also had a heat shock inducible transgene expressing Bam.
We then performed Polysome profiling of testes at 24, 48 and 72 hours PHS and created 3' end sequencing libraries from the free, 40S, 60s, 80S, 2-3 ribosomes, and 4+ ribosomes fractions for each dataset
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| Overall design |
3' end sequencing of testes from flies (genotype: bam-/-; hsBam) 0, 16, 24, 32, 48, 72 hours PHS and 3' end sequencing of Polysome Fractions (free, 40S, 60S, 80S, 2-3ribosomes, 4+ ribosomes) of testes from flies 24, 48 and 72 hours PHS
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| Contributor(s) |
Cameron B, Margaret F |
| Citation(s) |
36175033 |
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| Submission date |
May 13, 2024 |
| Last update date |
Aug 15, 2024 |
| Contact name |
Cameron W Berry |
| Organization name |
Stanford University
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| Department |
Developmental Biology
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| Lab |
Margaret Fuller
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| Street address |
279 W. Campus Drive, B300
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (48)
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| GSM8263656 |
Drosophila testes, 0h PHS, total lysate, Replicate 1 |
| GSM8263657 |
Drosophila testes,16h PHS, total lysate, Replicate 1 |
| GSM8263658 |
Drosophila testes, 24h PHS, total lysate, Replicate 1 |
| GSM8263659 |
Drosophila testes, 32h PHS, total lysate, Replicate 1 |
| GSM8263660 |
Drosophila testes, 48h PHS, total lysate, Replicate 1 |
| GSM8263661 |
Drosophila testes, 72h PHS, total lysate, Replicate 1 |
| GSM8263662 |
Drosophila testes, 24h PHS, Polysome fraction 'free', Replicate 1 |
| GSM8263663 |
Drosophila testes, 24h PHS, Polysome fraction '40S', Replicate 1 |
| GSM8263664 |
Drosophila testes, 24h PHS, Polysome fraction '60S', Replicate 1 |
| GSM8263665 |
Drosophila testes, 24h PHS, Polysome fraction '80S', Replicate 1 |
| GSM8263666 |
Drosophila testes, 24h PHS, Polysome fraction '2-3 ribosomes', Replicate 1 |
| GSM8263667 |
Drosophila testes, 24h PHS, Polysome fraction '4+ ribosomes', Replicate 1 |
| GSM8263668 |
Drosophila testes, 48h PHS, Polysome fraction 'free', Replicate 1 |
| GSM8263669 |
Drosophila testes, 48h PHS, Polysome fraction '40S', Replicate 1 |
| GSM8263670 |
Drosophila testes, 48h PHS, Polysome fraction '60S', Replicate 1 |
| GSM8263671 |
Drosophila testes, 48h PHS, Polysome fraction '80S', Replicate 1 |
| GSM8263672 |
Drosophila testes, 48h PHS, Polysome fraction '2-3 ribosomes', Replicate 1 |
| GSM8263673 |
Drosophila testes, 48h PHS, Polysome fraction '4+ ribosomes', Replicate 1 |
| GSM8263674 |
Drosophila testes, 72h PHS, Polysome fraction 'free', Replicate 1 |
| GSM8263675 |
Drosophila testes, 72h PHS, Polysome fraction '40S', Replicate 1 |
| GSM8263676 |
Drosophila testes, 72h PHS, Polysome fraction '60S', Replicate 1 |
| GSM8263677 |
Drosophila testes, 72h PHS, Polysome fraction '80S', Replicate 1 |
| GSM8263678 |
Drosophila testes, 72h PHS, Polysome fraction '2-3 ribosomes', Replicate 1 |
| GSM8263679 |
Drosophila testes, 72h PHS, Polysome fraction '4+ ribosomes', Replicate 1 |
| GSM8263680 |
Drosophila testes, 0h PHS, total lysate, Replicate 2 |
| GSM8263681 |
Drosophila testes,16h PHS, total lysate, Replicate 2 |
| GSM8263682 |
Drosophila testes, 24h PHS, total lysate, Replicate 2 |
| GSM8263683 |
Drosophila testes, 32h PHS, total lysate, Replicate 2 |
| GSM8263684 |
Drosophila testes, 48h PHS, total lysate, Replicate 2 |
| GSM8263685 |
Drosophila testes, 72h PHS, total lysate, Replicate 2 |
| GSM8263686 |
Drosophila testes, 24h PHS, Polysome fraction 'free', Replicate 2 |
| GSM8263687 |
Drosophila testes, 24h PHS, Polysome fraction '40S', Replicate 2 |
| GSM8263688 |
Drosophila testes, 24h PHS, Polysome fraction '60S', Replicate 2 |
| GSM8263689 |
Drosophila testes, 24h PHS, Polysome fraction '80S', Replicate 2 |
| GSM8263690 |
Drosophila testes, 24h PHS, Polysome fraction '2-3 ribosomes', Replicate 2 |
| GSM8263691 |
Drosophila testes, 24h PHS, Polysome fraction '4+ ribosomes', Replicate 2 |
| GSM8263692 |
Drosophila testes, 48h PHS, Polysome fraction 'free', Replicate 2 |
| GSM8263693 |
Drosophila testes, 48h PHS, Polysome fraction '40S', Replicate 2 |
| GSM8263694 |
Drosophila testes, 48h PHS, Polysome fraction '60S', Replicate 2 |
| GSM8263695 |
Drosophila testes, 48h PHS, Polysome fraction '80S', Replicate 2 |
| GSM8263696 |
Drosophila testes, 48h PHS, Polysome fraction '2-3 ribosomes', Replicate 2 |
| GSM8263697 |
Drosophila testes, 48h PHS, Polysome fraction '4+ ribosomes', Replicate 2 |
| GSM8263698 |
Drosophila testes, 72h PHS, Polysome fraction 'free', Replicate 2 |
| GSM8263699 |
Drosophila testes, 72h PHS, Polysome fraction '40S', Replicate 2 |
| GSM8263700 |
Drosophila testes, 72h PHS, Polysome fraction '60S', Replicate 2 |
| GSM8263701 |
Drosophila testes, 72h PHS, Polysome fraction '80S', Replicate 2 |
| GSM8263702 |
Drosophila testes, 72h PHS, Polysome fraction '2-3 ribosomes', Replicate 2 |
| GSM8263703 |
Drosophila testes, 72h PHS, Polysome fraction '4+ ribosomes', Replicate 2 |
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| Relations |
| BioProject |
PRJNA1111015 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267303_RAW.tar |
10.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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