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Series GSE267303 Query DataSets for GSE267303
Status Public on May 14, 2024
Title Developmentally regulated alternate 3’ end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary To determine the extent of alternative 3' end cleavage during Drosophila spermatogenesis, we performed 3' end sequencing of Drosophila testes from flies at different time points post heat shock (pHS) that were mutant for bam and also had a heat shock inducible transgene expressing Bam.
We then performed Polysome profiling of testes at 24, 48 and 72 hours PHS and created 3' end sequencing libraries from the free, 40S, 60s, 80S, 2-3 ribosomes, and 4+ ribosomes fractions for each dataset
 
Overall design 3' end sequencing of testes from flies (genotype: bam-/-; hsBam) 0, 16, 24, 32, 48, 72 hours PHS and 3' end sequencing of Polysome Fractions (free, 40S, 60S, 80S, 2-3ribosomes, 4+ ribosomes) of testes from flies 24, 48 and 72 hours PHS
 
Contributor(s) Cameron B, Margaret F
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 13, 2024
Last update date May 14, 2024
Contact name Cameron W Berry
Organization name Stanford University
Department Developmental Biology
Lab Margaret Fuller
Street address 279 W. Campus Drive, B300
City Stanford
State/province California
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (48)
GSM8263656 Drosophila testes, 0h PHS, total lysate, Replicate 1
GSM8263657 Drosophila testes,16h PHS, total lysate, Replicate 1
GSM8263658 Drosophila testes, 24h PHS, total lysate, Replicate 1
Relations
BioProject PRJNA1111015

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE267303_RAW.tar 10.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA

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