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Series GSE267402 Query DataSets for GSE267402
Status Public on May 17, 2024
Title Canid alphaherpesvirus 1 infection affects the gene expression and secretome profile of canine adipose-derived mesenchymal stem cells in vitro
Organism Canis lupus familiaris
Experiment type Expression profiling by RT-PCR
Summary Canine adipose -derived mesenchymal stem cells (cAD-MSCs) demonstrate promising tissue repair and regeneration capabilities. However, the procurement and preservation of these cells or their secreted factors for therapeutic applications pose a risk of viral contamination, and the consequences on cAD-MSCs remain unexplored. Consequently, this research sought to assess the impact of Canid alphaherpesvirus 1 on the functional attributes of cAD-MSCs, including gene expression profiles and secretome composition. To this end, abdominal fat tissue from twelve healthy dogs was harvested to isolate cAD-MSCs. These samples were tested for CHV contamination before introducing a wild-type CHV strain via serial passages. Following CHV infection, RT-PCR arrays and LC-MS/MS assessments enabled gene expression analysis and the secretome's proteomic landscape, respectively. The study established that the initial cAD-MSCs populations were devoid of CHV. Upon exposure to the virus, cAD-MSCs exhibited susceptibility, leading to notable modifications in gene expression and secretome profile. These changes reflected their intrinsic properties, such as stemness, differentiation potential, structural integrity, proliferative capacity, survival, directional migration, and immune-modulatory functions, depriving their regenerative efficacy . Despite the absence of pre-existing contamination, the outcomes underscore the imperative of routine viral screening prior to therapeutic use, particularly CHV. Moreover, these findings provide insights into the pathogenic mechanisms of CHV and the intricate interactions between canine stem cells and viruses.
 
Overall design Twelve biological replicates are studied in two different cell conditions, before and after infection with canine herpesvirus (Varicellovirus canidalpha1).
 
Contributor(s) Prišlin M, Brnić D
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Submission date May 14, 2024
Last update date May 22, 2024
Contact name Marina Prišlin
E-mail(s) prislin.marina@gmail.com
Organization name Croatian Veterinary Institute
Department Virology Department
Street address Savska cesta 148
City Zagreb
ZIP/Postal code 10000
Country Croatia
 
Platforms (1)
GPL34182 RT² ProfilerPCR Array for Dog Mesenchymal Stem Cells
Samples (24)
GSM8265215 Canine adipose-derived mesenchymal stem cells - biological replicate 1 - condition uninfected
GSM8265216 Canine adipose-derived mesenchymal stem cells - biological replicate 2 - condition uninfected
GSM8265217 Canine adipose-derived mesenchymal stem cells - biological replicate 3 - condition uninfected
Relations
BioProject PRJNA1111419

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE267402_processed_and_raw_data.xls.gz 41.2 Kb (ftp)(http) XLS

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