|
| Status |
Public on May 19, 2005 |
| Title |
Transcriptome effects of silencing a MAPK (SIPK) in tobacco |
| Platform organism |
Solanum tuberosum |
| Sample organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by array
|
| Summary |
Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group.
Series_weblink: http://www.tigr.org/tdb/potato
Keywords = potato, Biotic stress
Keywords: ordered
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| |
|
| Web link |
http://www.tigr.org/tdb/potato
|
| |
|
| Contributor(s) |
Ellis B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 17, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Li Zheng |
| E-mail(s) |
potato@tigr.org
|
| Phone |
301-795-7000
|
| Organization name |
The Institute for Genomic Research
|
| Department |
|
| Lab |
Robin Buell
|
| Street address |
9712 Medical Center Dr.
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1901 |
Potato 10k cDNA array version 2 |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA92203 |
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