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Series GSE267528 Query DataSets for GSE267528
Status Public on May 16, 2024
Title Unveiling the Immune Dynamics of Neisseria Persistent Oral Colonization
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Commensal bacteria are crucial in maintaining host physiological homeostasis, immune system development, and protection against pathogens. Despite their significance, the factors influencing persistent bacterial colonization and their impact on the host still need to be fully understood. Animal models have served as valuable tools to investigate these interactions, but most have limitations. The bacterial genus Neisseria, which includes both commensal and pathogenic species, has been studied from a pathogenicity to humans’ perspective, but lacks models that study immune responses in the context of long-term persistence. Neisseria musculi, a recently described natural commensal of mice, offers a unique opportunity to study long-term host-commensal interactions. In this study, for the first time we have used this model to study the transcriptional, phenotypic, and functional dynamics of immune cell signatures in the mucosal and systemic tissue of mice in response to Neisseria musculi colonization. We found key genes and pathways vital for immune homeostasis in palate tissue, validated by flow cytometry of immune cells from lung, blood and spleen. This study offers a novel avenue for advancing our understanding of host-bacteria dynamics and may provide a platform for developing efficacious interventions against mucosal persistence by pathogenic Neisseria.
Overall design Investigation of immune responses to Neisseria musculi colonization in a novel AJ mouse model. Hard palate samples were harvested 5 or 33 days post inoculation or mock inoculation, and used for total RNA extraction and RNAseq.
Contributor(s) Alles M, Gunasena M, Zia T, D’Mello A, Bhattarai S, Mulhern W, Terry L, Scherger T, Wijeratne S, Singh S, Wijeratne AJ, Kasturiratna D, Tettelin H, Weyand NJ, Liyanage NP
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Submission date May 15, 2024
Last update date May 16, 2024
Contact name Suvarna Nadendla
Organization name University of Maryland School of Medicine
Department Institute for Genome Sciences
Street address 670 W.Baltimore Street
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM8268231 AJ_Palate_Bottom1_D5colonized_biol_rep1
GSM8268232 AJ_Palate_Bottom2_D33colonized_biol_rep1
GSM8268233 AJ_Palate_Bottom3_D5mock_biol_rep1
BioProject PRJNA1111780

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Supplementary file Size Download File type/resource
GSE267528_Neisseria_musculi_AJ_mouse_model_InfectImmun2024_raw_counts.txt.gz 907.6 Kb (ftp)(http) TXT
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