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Series GSE267817 Query DataSets for GSE267817
Status Public on May 25, 2024
Title Placental co-transcriptional activator Vestigial-like 1 (VGLL1) drives tumorigenesis via increasing transcription of proliferation and invasion genes [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Vestigial-like 1 (VGLL1) is a co-transcriptional activator that binds to TEA domain-containing transcription factors (TEADs). Its expression is upregulated in a variety of aggressive cancer types, including pancreatic and basal-like breast cancer, and increased transcription of VGLL1 is strongly correlated with poor prognosis and decreased overall patient survival. In normal tissues, VGLL1 is most highly expressed within placental trophoblast cells, which share the common attributes of rapid cellular proliferation and invasion with tumor cells. The impact of VGLL1 in cancer has not been fully elucidated and no VGLL1-targeted therapy currently exists. The aim of this study was to evaluate the cellular function and downstream genomic targets of VGLL1 in placental, pancreatic, and breast cancer cells. Functional assays were employed to assess the role of VGLL1 in cellular invasion and proliferation, and ChIP-seq and RNA-seq assays were performed to identify VGLL1 target genes and potential impact using pathway analysis. ChIP-seq analysis identified eight transcription factors with a VGLL1-binding motif that were common between all three cell types, including TEAD1-4, AP-1, and GATA6, and revealed ~3,000 shared genes with which VGLL1 interacts. Furthermore, increased VGLL1 expression led to an enhancement of cell invasion and proliferation, which was supported by RNA-seq analysis showing transcriptional changes in several genes known to be involved in these processes. This work expands our mechanistic understanding of VGLL1 function in tumor cells and provides a strong rationale for developing VGLL1-targeted therapies for treating cancer patients.
 
Overall design ChiP-seq analysis of VGLL1 for different cancer cells: PANC10.05, BT20, and Bewo cells.
 
Contributor(s) Sonnemann H, Lizee G
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Submission date May 18, 2024
Last update date May 25, 2024
Contact name Heather Sonnemann
E-mail(s) HMSonnemann@mdanderson.org
Organization name MD Anderson Cancer Center
Street address 7455 Fannin St
City Houston
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM8279244 BT20 input
GSM8279245 BT20 VGLL1
GSM8279246 PANC10.05 input
Relations
BioProject PRJNA1113182

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Supplementary file Size Download File type/resource
GSE267817_RAW.tar 814.4 Mb (http)(custom) TAR (of BW)
GSE267817_VGLL1_ucsctracks_bw.bed.gz 1.4 Mb (ftp)(http) BED
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